Molecular characterization of environmental Cryptococcus neoformans VNII isolates in Jos, Plateau State, Nigeria

J Mycol Med. 2016 Dec;26(4):306-311. doi: 10.1016/j.mycmed.2016.04.001. Epub 2016 May 12.

Abstract

Cryptococcus neoformans and Cryptococcus gattii are encapsulated yeasts able to cause fatal neurological infections in both human and other mammals. Cryptococcosis is the most common fungal infection of the central nervous system and has a huge burden in sub-Saharan Africa and South East Asia. Bird excreta are considered an environmental reservoir for C. neoformans in urban areas, therefore a study aimed at isolating and characterizing this yeast is important in disease management. In this study, one hundred samples of pigeon droppings were collected in Jos, Plateau State, Nigeria. C. neoformans was isolated from three samples and initially identified using standard phenotypic and biochemical tests. Molecular analysis revealed that all three isolates belonged to C. neoformans genotype VNII, mating type α and were assigned to the sequence type ST43 by multilocus sequence typing analysis. This study reports, for the first time, the molecular characterization of C. neoformans in Nigeria, where little is still known about the environmental distribution of the genotypes, serotypes and mating types of this important human pathogen.

Keywords: Cryptococcus neoformans; Environment; Environnement; Mating types; Molecular types; Nigeria.

MeSH terms

  • Animals
  • Antifungal Agents / pharmacology
  • Columbidae / microbiology
  • Cryptococcosis / microbiology
  • Cryptococcus neoformans / genetics*
  • Cryptococcus neoformans / isolation & purification*
  • DNA, Fungal
  • Environmental Microbiology*
  • Feces / microbiology
  • Genes, Mating Type, Fungal
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Typing / methods
  • Mycological Typing Techniques
  • Nigeria

Substances

  • Antifungal Agents
  • DNA, Fungal