Spread of Plasmids Carrying Multiple GES Variants

Gaelle Cuzon; Pierre Bogaerts; Caroline Bauraing; Te-Din Huang; Rémy A Bonnin; Youri Glupczynski; Thierry Naas

doi:10.1128/AAC.00360-16

Spread of Plasmids Carrying Multiple GES Variants

Antimicrob Agents Chemother. 2016 Jul 22;60(8):5040-3. doi: 10.1128/AAC.00360-16. Print 2016 Aug.

Authors

Gaelle Cuzon¹, Pierre Bogaerts², Caroline Bauraing², Te-Din Huang², Rémy A Bonnin³, Youri Glupczynski², Thierry Naas⁴

Affiliations

¹ Bactériologie-Hygiene Unit, APHP, Bicêtre Hospital, Le Kremlin-Bicêtre, France Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicetre, France French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France.
² Belgian National Reference Centre for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
³ Bactériologie-Hygiene Unit, APHP, Bicêtre Hospital, Le Kremlin-Bicêtre, France Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicetre, France French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France Evolution and Ecology of Resistance to Antibiotics (EERA) Unit, Institut Pasteur-APHP-Université Paris Sud, Le Kremlin-Bicetre, France.
⁴ Bactériologie-Hygiene Unit, APHP, Bicêtre Hospital, Le Kremlin-Bicêtre, France Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicetre, France French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France Evolution and Ecology of Resistance to Antibiotics (EERA) Unit, Institut Pasteur-APHP-Université Paris Sud, Le Kremlin-Bicetre, France thierry.naas@bct.aphp.fr.

Abstract

Five GES-producing Enterobacteriaceae isolates that displayed an extended-spectrum β-lactamase (ESBL) phenotype harbored two GES variants: GES-7 ESBL and GES-6 carbapenemase. In all isolates, the two GES alleles were located on the same integron that was inserted into an 80-kb IncM1 self-conjugative plasmid. Whole-genome sequencing suggested in vivo horizontal gene transfer of the plasmid along with clonal diffusion of Enterobacter cloacae To our knowledge, this is the first description in Europe of clustered Enterobacteriaceae isolates carrying two GES β-lactamases, of which one has extended activity toward carbapenems.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Enterobacter cloacae / drug effects
Enterobacter cloacae / enzymology
Enterobacter cloacae / genetics
Enterobacteriaceae / drug effects*
Enterobacteriaceae / enzymology*
Enterobacteriaceae / genetics
Gene Transfer, Horizontal / genetics
Microbial Sensitivity Tests
Plasmids / genetics*
beta-Lactamases / genetics
beta-Lactamases / metabolism*

Substances

Anti-Bacterial Agents
Bacterial Proteins
beta-Lactamases
carbapenemase

Grants and funding

This work, including the efforts of Thierry Naas, was funded by University Paris 11 (EA7361), by LabEx LERMIT (ANR-10-LABX-33), and by the European Commission (EC) (MAGIC-BULLET FP7/HEALTH-F3-2001-27823). This work, including the efforts of Youri Glupczynski, was funded by Fondation Mont-Godinne.