DNA Triplexes-Guided Assembly of G-Quadruplexes for Constructing Label-free Fluorescent Logic Gates

Lijun Xu; Shanni Hong; Xiaoqiang Shen; Lu Zhou; Jine Wang; Jianye Zhang; Renjun Pei

doi:10.1002/asia.201600626

DNA Triplexes-Guided Assembly of G-Quadruplexes for Constructing Label-free Fluorescent Logic Gates

Chem Asian J. 2016 Jul 5;11(13):1892-5. doi: 10.1002/asia.201600626. Epub 2016 Jun 6.

Authors

Lijun Xu^{1

2}, Shanni Hong^{1

2}, Xiaoqiang Shen^{1

3}, Lu Zhou¹, Jine Wang¹, Jianye Zhang⁴, Renjun Pei⁵

Affiliations

¹ Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ School of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, China.
⁴ School of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, China. zhangjy@zzu.edu.cn.
⁵ Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China. rjpei2011@sinano.ac.cn.

PMID: 27224871
DOI: 10.1002/asia.201600626

Abstract

Assembly of G-quadruplexes guided by DNA triplexes in a controlled manner is achieved for the first time. The folding of triplex sequences in acidic conditions brings two separated guanine-rich sequences together and subsequently a G-quadruplex structure is formed in the presence of K(+) . Based on this novel platform, label-free fluorescent logic gates, such as AND, INHIBIT, and NOR, are constructed with ions as input and the fluorescence of a G-quadruplex-specific fluorescent probe NMM as output.

Keywords: DNA; G-quadruplexes; fluorescent probes; self-assembly; triplex DNA.

MeSH terms

Base Sequence
Computers, Molecular
DNA / chemistry*
Fluorescent Dyes / chemistry*
G-Quadruplexes*
Nucleic Acid Conformation
Spectrometry, Fluorescence

Substances

Fluorescent Dyes
triplex DNA
DNA