Molecular characterization of the first G24P[14] rotavirus strain detected in humans

M Leanne Ward; Slavica Mijatovic-Rustempasic; Sunando Roy; Kunchala Rungsrisuriyachai; Julie A Boom; Leila C Sahni; Carol J Baker; Marcia A Rench; Mary E Wikswo; Daniel C Payne; Umesh D Parashar; Michael D Bowen

doi:10.1016/j.meegid.2016.05.033

Molecular characterization of the first G24P[14] rotavirus strain detected in humans

Infect Genet Evol. 2016 Sep:43:338-42. doi: 10.1016/j.meegid.2016.05.033. Epub 2016 May 26.

Authors

Affiliations

¹ Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: mrw0@cdc.gov.
² Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: hsr7@cdc.gov.
³ Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: sunando@gmail.com.
⁴ Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: Torekunchala@gmail.com.
⁵ Texas Children's Hospital, Houston, TX, USA; Baylor College of Medicine, Houston, TX, USA. Electronic address: jaboom@texaschildrens.org.
⁶ Texas Children's Hospital, Houston, TX, USA. Electronic address: lcsahni@texaschildrens.org.
⁷ Texas Children's Hospital, Houston, TX, USA; Baylor College of Medicine, Houston, TX, USA. Electronic address: cbaker@bcm.edu.
⁸ Texas Children's Hospital, Houston, TX, USA; Baylor College of Medicine, Houston, TX, USA. Electronic address: mrench@bcm.edu.
⁹ Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: ezq1@cdc.gov.
¹⁰ Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: dvp6@cdc.gov.
¹¹ Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: uap2@cdc.gov.
¹² Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: mkb6@cdc.gov.

Abstract

Here we report the genome of a novel rotavirus A (RVA) strain detected in a stool sample collected during routine surveillance by the Centers for Disease Control and Prevention's New Vaccine Surveillance Network. The strain, RVA/human-wt/USA/2012741499/2012/G24P[14], has a genomic constellation of G24-P[14]-I2-R2-C2-M2-A3-N2-T9-E2-H3. The VP2, VP3, VP7 and NSP3 genes cluster phylogenetically with bovine strains. The other genes occupy mixed clades containing animal and human strains. Strain RVA/human-wt/USA/2012741499/2012/G24P[14] most likely is the product of interspecies transmission and reassortment events. This is the second report of the G24 genotype and the first report of the G24P[14] genotype combination in humans.

Keywords: Bovine; G24P[14]; Reassortment; Rotavirus.

Published by Elsevier B.V.

Publication types

Case Reports
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Cattle
Child, Preschool
Genome, Viral*
Genotype*
High-Throughput Nucleotide Sequencing
Humans
Male
Phylogeny*
Reassortant Viruses / classification
Reassortant Viruses / genetics*
Rotavirus / classification
Rotavirus / genetics*
Rotavirus Infections / virology
Texas
Viral Nonstructural Proteins / genetics*

Substances

Viral Nonstructural Proteins

Grants and funding

CC999999/Intramural CDC HHS/United States