Genome-scale CRISPR pooled screens

Anal Biochem. 2017 Sep 1:532:95-99. doi: 10.1016/j.ab.2016.05.014. Epub 2016 Jun 1.

Abstract

Genome editing technologies such as clustered regularly interspaced short palindromic repeats (CRISPR) systems have ushered in a new era of targeted DNA manipulation. The easy programmability of CRISPR using short oligonucleotides enables rapid synthesis of large-scale libraries for functional genetic screens. Here we present fundamental concepts and methods for pooled CRISPR screens and review biological results from recent genome-scale loss-of-function and gain-of-function screens. We also discuss new frontiers in pooled screens, including novel effector domains for functional screens and applications in the noncoding genome.

Keywords: CRISPR; Cancer; Cas9; Genetic screens; Genome engineering; Noncoding.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • CRISPR-Cas Systems / genetics*
  • Gene Editing / methods*
  • Gene Targeting / methods*
  • Genetic Engineering*
  • Genome, Human*
  • Humans
  • RNA, Guide, CRISPR-Cas Systems / genetics*

Substances

  • RNA, Guide, CRISPR-Cas Systems