A restricted period for formation of outer subventricular zone defined by Cdh1 and Trnp1 levels

Nat Commun. 2016 Jun 6:7:11812. doi: 10.1038/ncomms11812.

Abstract

The outer subventricular zone (OSVZ) is a germinal layer playing key roles in the development of the neocortex, with particular relevance in gyrencephalic species such as human and ferret, where it contains abundant basal radial glia cells (bRGCs) that promote cortical expansion. Here we identify a brief period in ferret embryonic development when apical RGCs generate a burst of bRGCs that become founders of the OSVZ. After this period, bRGCs in the OSVZ proliferate and self-renew exclusively locally, thereby forming a self-sustained lineage independent from the other germinal layers. The time window for the brief period of OSVZ bRGC production is delineated by the coincident downregulation of Cdh1 and Trnp1, and their upregulation reduces bRGC production and prevents OSVZ seeding. This mechanism in cortical development may have key relevance in brain evolution and disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cdh1 Proteins / genetics
  • Cdh1 Proteins / metabolism*
  • Cell Lineage / genetics
  • Cell Proliferation / genetics
  • Cell Self Renewal
  • Ependymoglial Cells / metabolism*
  • Ferrets
  • Gene Expression Profiling / methods
  • Gene Expression Regulation, Developmental
  • Lateral Ventricles / cytology
  • Lateral Ventricles / embryology
  • Lateral Ventricles / metabolism*
  • Neocortex / cytology
  • Neocortex / embryology
  • Neocortex / metabolism
  • Neurogenesis / genetics
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Time-Lapse Imaging / methods

Substances

  • Cdh1 Proteins
  • Nuclear Proteins