H-ferritin and CD68(+) /H-ferritin(+) monocytes/macrophages are increased in the skin of adult-onset Still's disease patients and correlate with the multi-visceral involvement of the disease

Clin Exp Immunol. 2016 Oct;186(1):30-8. doi: 10.1111/cei.12826. Epub 2016 Jul 28.

Abstract

Adult-onset Still's disease (AOSD) patients may show an evanescent salmon-pink erythema appearing during febrile attacks and reducing without fever. Some patients may experience this eruption for many weeks. During AOSD, exceptionally high serum levels of ferritin may be observed; it is an iron storage protein composed of 24 subunits, heavy (H) subunits and light (L) subunits. The ferritin enriched in L subunits (L-ferritin) and the ferritin enriched in H subunits (H-ferritin) may be observed in different tissues. In this work, we aimed to investigate the skin expression of both H-and L-ferritin and the number of macrophages expressing these molecules from AOSD patients with persistent cutaneous lesions. We observed an increased expression of H-ferritin in the skin, associated with an infiltrate in the biopsies obtained from persistent cutaneous lesions of AOSD patients. Furthermore, a positive correlation between H-ferritin skin levels as well as the number of CD68(+) /H-ferritin(+) cells and the multi-visceral involvement of the disease was observed. Our data showed an increased expression of H-ferritin in the skin of AOSD patients, associated with a strong infiltrate of CD68(+) /H-ferritin(+) cells. Furthermore, a correlation between the levels of H-ferritin as well as of the number of CD68(+) /H-ferritin(+) cells and the multi-visceral involvement of the disease was observed.

Keywords: adult-onset Still's disease; dermal immune system; ferritin; hyperferritinaemic syndrome; macrophage.

MeSH terms

  • Antigens, CD / metabolism
  • Antigens, Differentiation, Myelomonocytic / metabolism
  • Apoferritins / genetics
  • Apoferritins / metabolism*
  • Biomarkers
  • Biopsy
  • Cytokines / metabolism
  • Female
  • Gene Expression
  • Humans
  • Inflammation Mediators / metabolism
  • Leukocytes, Mononuclear / immunology
  • Leukocytes, Mononuclear / metabolism
  • Macrophages / immunology
  • Macrophages / metabolism*
  • Male
  • Monocytes / immunology
  • Monocytes / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Skin / immunology*
  • Skin / metabolism*
  • Skin / pathology
  • Still's Disease, Adult-Onset / diagnosis
  • Still's Disease, Adult-Onset / immunology*
  • Still's Disease, Adult-Onset / metabolism*

Substances

  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • Biomarkers
  • CD68 antigen, human
  • Cytokines
  • Inflammation Mediators
  • RNA, Messenger
  • Apoferritins