Serum Angiopoietin-Like Protein 2 Is a Novel Risk Factor for Cardiovascular Disease in the Community: The Hisayama Study

Arterioscler Thromb Vasc Biol. 2016 Aug;36(8):1686-91. doi: 10.1161/ATVBAHA.116.307291. Epub 2016 Jun 30.

Abstract

Objective: Angiopoietin-like protein 2 (ANGPTL2), a proinflammatory mediator, has been reported to accelerate the development of insulin resistance, endothelial dysfunction, and atherosclerosis in mice. However, no cohort studies have examined the relationship between serum ANGPTL2 levels and the development of cardiovascular disease (CVD) in a general population.

Approach and results: A total of 3005 community-dwelling Japanese aged ≥40 years without a history of CVD were divided into 4 groups according to the quartiles of serum ANGPTL2 concentrations (Q1, lowest and Q4, highest) and followed up for 10 years. The hazards ratios and their 95% confidence intervals for the development of CVD (coronary heart disease or stroke) were estimated using a Cox proportional hazards model. During the follow-up, 219 first-ever CVD events were observed. The risk of CVD increased significantly with elevating ANGPTL2 levels after adjustment for age, sex, serum total cholesterol, use of lipid-lowering agents, ECG abnormalities, smoking habits, alcohol intake, and regular exercise (hazards ratios [95% confidence interval], Q1, 1.00 [reference]; Q2, 1.27 [0.80-2.04]; Q3, 1.48 [0.95-2.32]; and Q4, 1.85 [1.20-2.85]; P=0.003 for trend). After additional adjustment for metabolic syndrome components and serum high-sensitivity C-reactive protein levels as an inflammatory marker, the association was attenuated but remained significant (hazards ratios [95% confidence interval], Q1, 1.00 [reference]; Q2, 1.21 [0.76-1.94]; Q3, 1.38 [0.87-2.17]; and Q4, 1.66 [1.05-2.60]; P=0.02 for trend).

Conclusions: Our findings suggest that elevated serum ANGPTL2 levels are a novel risk factor for the development of CVD in the general population. This association is partially mediated by metabolic disorders and inflammation.

Keywords: angiopoietin; cardiovascular disease; cohort study; inflammation; stroke.

MeSH terms

  • Age Distribution
  • Aged
  • Angiopoietin-Like Protein 2
  • Angiopoietin-like Proteins
  • Angiopoietins / blood*
  • Biomarkers / blood
  • C-Reactive Protein / metabolism
  • Cardiovascular Diseases / blood*
  • Cardiovascular Diseases / diagnosis
  • Cardiovascular Diseases / epidemiology*
  • Female
  • Humans
  • Incidence
  • Inflammation / blood
  • Inflammation / epidemiology
  • Japan / epidemiology
  • Linear Models
  • Logistic Models
  • Male
  • Metabolic Diseases / blood
  • Metabolic Diseases / epidemiology
  • Middle Aged
  • Prognosis
  • Proportional Hazards Models
  • Prospective Studies
  • Risk Assessment
  • Risk Factors
  • Sex Distribution
  • Time Factors
  • Up-Regulation

Substances

  • ANGPTL2 protein, human
  • Angiopoietin-Like Protein 2
  • Angiopoietin-like Proteins
  • Angiopoietins
  • Biomarkers
  • C-Reactive Protein