Abstract
In this paper, we report the conjugation of the humanized monoclonal antibody nimotuzumab with cisplatin-loaded liposomes and the in vitro evaluation of its affinity for tumor cells. The conjugation procedure was performed through derivatization of nimotuzumab with N-succinimidyl S-acetylthioacetate (SATA) followed by a covalent attachment with maleimide groups at the end of PEG-DSPE chains located at the membrane of pre-formed liposomes. Confocal microscopy was performed to evaluate the immunoliposome affinity for EGFR antigens from human epidermoid carcinoma (A-431) and normal lung (MRC-5) cell lines. Results showed that the procedures implemented in this work do not affect the capability of the nimotuzumab-immunoliposomes to recognize the tumor cells, which overexpress the EGFR antigens.
Keywords:
Drug carriers; EGFR; immunoliposomes; monoclonal antibody; tumor cell targeting.
MeSH terms
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Antibodies, Monoclonal, Humanized / chemistry*
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Antibodies, Monoclonal, Humanized / metabolism
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Antineoplastic Agents / chemistry*
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Antineoplastic Agents / immunology
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Antineoplastic Agents / pharmacology
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Carcinoma, Squamous Cell / metabolism
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Cell Line, Tumor
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Cisplatin / administration & dosage
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Cisplatin / chemistry*
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Drug Carriers / chemistry*
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Drug Liberation
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Drug Stability
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Green Fluorescent Proteins / immunology
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Humans
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Liposomes / chemical synthesis*
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Lung / metabolism
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Lung Neoplasms / metabolism
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Maleimides / chemistry
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Particle Size
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Phosphatidylethanolamines / chemistry
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Polyethylene Glycols / chemistry
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Succinimides / chemistry
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Sulfides / chemistry
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Surface Properties
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Tumor Cells, Cultured
Substances
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Antibodies, Monoclonal, Humanized
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Antineoplastic Agents
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Drug Carriers
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Liposomes
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Maleimides
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Phosphatidylethanolamines
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Succinimides
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Sulfides
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enhanced green fluorescent protein
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polyethylene glycol-distearoylphosphatidylethanolamine
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Green Fluorescent Proteins
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maleimide
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Polyethylene Glycols
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nimotuzumab
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N-hydroxysuccinimide S-acetylthioacetate
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Cisplatin