Interleukin-6 and associated cytokine responses to an acute bout of high-intensity interval exercise: the effect of exercise intensity and volume

Appl Physiol Nutr Metab. 2016 Aug;41(8):803-8. doi: 10.1139/apnm-2015-0640. Epub 2016 Mar 18.

Abstract

Acute increases in interleukin (IL)-6 following prolonged exercise are associated with the induction of a transient anti-inflammatory state (e.g., increases in IL-10) that is partly responsible for the health benefits of regular exercise. The purposes of this study were to investigate the IL-6-related inflammatory response to high-intensity interval exercise (HIIE) and to determine the impact of exercise intensity and volume on this response. Ten participants (5 males and 5 females) completed 3 exercise bouts of contrasting intensity and volume (LOW, MOD, and HIGH). The HIGH protocol was based upon standard HIIE protocols, while the MOD and LOW protocols were designed to enable a comparison of exercise intensity and volume with a fixed duration. Inflammatory cytokine concentrations were measured in plasma (IL-6, IL-10) and also determined the level of gene expression (IL-6, IL-10, and IL-4R) in peripheral blood. The plasma IL-6 response to exercise (reported as fold changes) was significantly greater in HIGH (2.70 ± 1.51) than LOW (1.40 ± 0.32) (P = 0.04) and was also positively correlated to the mean exercise oxygen uptake (r = 0.54, P < 0.01). However, there was no change in anti-inflammatory IL-10 or IL-4R responses in plasma or at the level of gene expression. HIIE caused a significant increase in IL-6 and was greater than that seen in low-intensity exercise of the same duration. The increases in IL-6 were relatively small in magnitude, and appear to have been insufficient to induce the acute systemic anti-inflammatory effects, which are evident following longer duration exercise.

Keywords: HIIT; anti-inflammatoires; anti-inflammatory; cytokines; exercice physique; exercise; high-intensity; intensité élevée; interval; intervalle.

MeSH terms

  • Adult
  • Diet
  • Exercise / physiology*
  • Female
  • Gene Expression Regulation
  • High-Intensity Interval Training*
  • Humans
  • Interleukin-10 / blood
  • Interleukin-10 / genetics
  • Interleukin-6 / blood*
  • Interleukin-6 / genetics
  • Linear Models
  • Male
  • Oxygen Consumption
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Interleukin-6 / blood
  • Young Adult

Substances

  • IL10 protein, human
  • IL6 protein, human
  • IL6R protein, human
  • Interleukin-6
  • RNA, Messenger
  • Receptors, Interleukin-6
  • Interleukin-10