High-Fat-Diet Intake Enhances Cerebral Amyloid Angiopathy and Cognitive Impairment in a Mouse Model of Alzheimer's Disease, Independently of Metabolic Disorders

J Am Heart Assoc. 2016 Jun 13;5(6):e003154. doi: 10.1161/JAHA.115.003154.

Abstract

Background: The high-fat Western diet is postulated to be associated with the onset and progression of Alzheimer's disease (AD). However, the role of high-fat-diet consumption in AD pathology is unknown. This study was undertaken to examine the role of high-fat-diet intake in AD.

Methods and results: 5XFAD mice, a useful mouse model of AD, and control wild-type mice were fed (1) high-fat diet or (2) control diet for 10 weeks. The effects on cerebral AD pathology, cognitive function, and metabolic parameters were compared between each group of mice. High-fat diet significantly enhanced cerebrovascular β-amyloid (Aβ) deposition (P<0.05) and impaired cognitive function (P<0.05) in 5XFAD mice, but not in wild-type mice. High-fat diet enhanced hippocampal oxidative stress (P<0.05) and NADPH oxidase subunits, gp91(phox) (P<0.01) and p22(phox) (P<0.01) in 5XFAD mice, but not in wild-type mice. Furthermore, high-fat diet reduced cerebral occludin (P<0.05) in 5XFAD mice, but not in wild-type mice. Thus, 5XFAD mice exhibited greater susceptibility to high-fat diet than wild-type mice regarding cerebrovascular injury and cognitive impairment. On the other hand, 5XFAD mice fed high-fat diet exhibited much less increase in body weight, white adipose tissue weight, and adipocyte size than their wild-type counterparts. High-fat diet significantly impaired glucose tolerance in wild-type mice but not in 5XFAD mice. Thus, 5XFAD mice had much less susceptibility to high-fat-diet-induced metabolic disorders than wild-type mice.

Conclusions: High-fat diet, independently of metabolic disorders, significantly promotes the progression of AD-like pathology through enhancement of cerebral amyloid angiopathy and oxidative stress.

Keywords: brain; cerebrovascular disorders; nutrition; obesity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / pathology
  • Alzheimer Disease / etiology*
  • Alzheimer Disease / pathology
  • Alzheimer Disease / physiopathology
  • Amyloid / metabolism
  • Animals
  • Blood Glucose / metabolism
  • Blood Pressure / physiology
  • Body Weight / physiology
  • Brain / pathology
  • Brain-Derived Neurotrophic Factor / metabolism
  • Catalase / metabolism
  • Cerebral Amyloid Angiopathy / etiology*
  • Cerebral Amyloid Angiopathy / pathology
  • Cerebral Amyloid Angiopathy / physiopathology
  • Cerebrovascular Disorders / etiology
  • Cerebrovascular Disorders / pathology
  • Cerebrovascular Disorders / physiopathology
  • Cognitive Dysfunction / etiology*
  • Cognitive Dysfunction / pathology
  • Cognitive Dysfunction / physiopathology
  • Cyclooxygenase 2 / metabolism
  • Diet, High-Fat / adverse effects*
  • Drinking / physiology
  • Eating / physiology
  • Glucose Tolerance Test
  • Male
  • Maze Learning
  • Mice, Inbred Strains
  • NADPH Oxidases / metabolism
  • Occludin / metabolism
  • Organ Size / physiology
  • Oxidative Stress / physiology
  • Superoxide Dismutase / metabolism
  • Urine / physiology

Substances

  • Amyloid
  • Blood Glucose
  • Brain-Derived Neurotrophic Factor
  • Occludin
  • Catalase
  • Cyclooxygenase 2
  • Superoxide Dismutase
  • NADPH Oxidases