Beneficial cyanobacteria and eubacteria synergistically enhance bioavailability of soil nutrients and yield of okra

Mallappa Manjunath; Amrita Kanchan; Kunal Ranjan; Siddarthan Venkatachalam; Radha Prasanna; Balasubramanian Ramakrishnan; Firoz Hossain; Lata Nain; Yashbir Singh Shivay; Awadhesh Bahadur Rai; Bijendra Singh

doi:10.1016/j.heliyon.2016.e00066

Beneficial cyanobacteria and eubacteria synergistically enhance bioavailability of soil nutrients and yield of okra

Heliyon. 2016 Feb 8;2(2):e00066. doi: 10.1016/j.heliyon.2016.e00066. eCollection 2016 Feb.

Affiliations

¹ Division of Crop Protection, ICAR-Indian Institute of Vegetable Research, Jakhini, Sahanshapur, Varanasi, 221305, Uttar Pradesh, India.
² Division of Microbiology, ICAR-Indian Agricultural Research Institute (IARI), New Delhi, 110012, India.
³ Division of Genetics, ICAR-Indian Agricultural Research Institute (IARI), New Delhi, 110012, India.
⁴ Division of Agronomy, ICAR-Indian Agricultural Research Institute (IARI), New Delhi, 110012, India.

Abstract

Microorganisms in the rhizosphere mediate the cycling of nutrients, their enhanced mobilisation and facilitate their uptake, leading to increased root growth, biomass and yield of plants. We examined the promise of beneficial cyanobacteria and eubacteria as microbial inoculants, applied singly or in combination as consortia or biofilms, to improve growth and yields of okra. Interrelationships among the microbial activities and the micro/macro nutrient dynamics in soils and okra yield characteristics were assessed along with the changes in the soil microbiome. A significant effect of microbial inoculation on alkaline phosphatase activity was recorded both at the mid-crop and harvest stages. Microbial biomass carbon values were highest due to the Anabaena sp. - Providencia sp. (CR1 + PR3) application. The yield of okra ranged from 444.6-478.4 g(-1) plant and a positive correlation (0.69) recorded between yield and root weight. The application of Azotobacter led to the highest root weight and yield. The concentration of Zn at mid-crop stage was 60-70% higher in the Azotobacter sp. and Calothrix sp. inoculated soils, as compared to uninoculated control. Iron concentration in soil was more than 2-3 folds higher than control at the mid-crop stage, especially due to the application of Anabaena-Azotobacter biofilm and Azotobacter sp. Both at the mid-crop and harvest stages, the PCR-DGGE profiles of eubacterial communities were similar among the uninoculated control, the Anabaena sp. - Providencia sp. (CW1 + PW5) and the Anabaena-Azotobacter biofilm treatments. Although the profiles of the Azotobacter, Calothrix and CR1 + PR3 treatments were identical at these stages of growth, the profile of CR1 + PR3 was clearly distinguishable. The performance of the inoculants, particularly Calothrix (T6) and consortium of Anabaena and Providencia (CR1 + PR3; T5), in terms of microbiological and nutrient data, along with generation of distinct PCR-DGGE profiles suggested their superiority and emphasized the utility of combining microbiological and molecular tools in the selection of effective microbial inoculants.

Keywords: Agriculture; Agronomy; Ecology; Horticulture; Microbiology; Soil science.