Irisolidone attenuates ethanol-induced gastric injury in mice by inhibiting the infiltration of neutrophils

Mol Nutr Food Res. 2017 Feb;61(2). doi: 10.1002/mnfr.201600517. Epub 2016 Oct 5.

Abstract

Scope: This study was designed to determine whether irisolidone and its glycoside kakkalide, which are the major constituents of the flower of Pueraria lobata (Kudzu) can attenuate ethanol-induced gastritic injury in mice.

Methods and results: Irisolidone and kakkalide inhibited IL-8 secretion and NF-κB activation in lipopolysaccharide-stimulated KATO III cells. Therefore, we investigated their protective effects against ethanol-induced gastric injury in mice. Pretreatment with kakkalide or irisolidone decreased the area of hemorrhagic ulcerative lesions caused by ethanol and suppressed stomach myeloperoxidase activity, CXCL4 secretion, and NF-κB activation. The ameliorating effect of irisolidone was more potent than that of kakkalide.

Conclusion: Irisolidone may attenuate ethanol-induced gastritis by inhibiting the infiltration of immune cells, particularly neutrophils, through the regulation of CXCL-4 or IL-8 secretion.

Keywords: Ethanol; Gastritis; Irisolidone; Kudzu; Neutrophil.

MeSH terms

  • Animals
  • Cell Line
  • Ethanol / adverse effects*
  • Flavonoids / pharmacology*
  • Gastritis / chemically induced
  • Gastritis / drug therapy*
  • Gastritis / metabolism
  • Glycosides / pharmacology
  • Interleukin-8 / metabolism
  • Isoflavones / pharmacology
  • Lipopolysaccharides / pharmacology
  • Male
  • Mice, Inbred ICR
  • NF-kappa B / metabolism
  • Neutrophils / drug effects*
  • Peroxidase / metabolism
  • Platelet Factor 4 / metabolism
  • Protective Agents / pharmacology
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Flavonoids
  • Glycosides
  • Interleukin-8
  • Isoflavones
  • Lipopolysaccharides
  • NF-kappa B
  • Protective Agents
  • Tumor Necrosis Factor-alpha
  • irisolidone
  • Platelet Factor 4
  • Ethanol
  • kakkalide
  • Peroxidase