Serum Procalcitonin and Peripheral Venous Lactate for Predicting Dengue Shock and/or Organ Failure: A Prospective Observational Study

PLoS Negl Trop Dis. 2016 Aug 26;10(8):e0004961. doi: 10.1371/journal.pntd.0004961. eCollection 2016 Aug.

Abstract

Background: Currently, there are no biomarkers that can predict the incidence of dengue shock and/or organ failure, although the early identification of risk factors is important in determining appropriate management to reduce mortality. Therefore, we sought to determine the factors associated with dengue shock and/or organ failure and to evaluate the prognostic value of serum procalcitonin (PCT) and peripheral venous lactate (PVL) levels as biomarkers of dengue shock and/or organ failure.

Methodology/principal findings: A prospective observational study was conducted among adults hospitalized for confirmed viral dengue infection at the Hospital for Tropical Diseases in Bangkok, Thailand between October 2013 and July 2015. Data, including baseline characteristics, clinical parameters, laboratory findings, serum PCT and PVL levels, management, and outcomes, were recorded on pre-defined case report forms. Of 160 patients with dengue, 128 (80.0%) patients had dengue without shock or organ failure, whereas 32 (20.0%) patients developed dengue with shock and/or organ failure. Using a stepwise multivariate logistic regression analysis, PCT ≥0.7 ng/mL (odds ratio [OR]: 4.80; 95% confidence interval [CI]: 1.60-14.45; p = 0.005) and PVL ≥2.5 mmol/L (OR: 27.99, 95% CI: 8.47-92.53; p <0.001) were independently associated with dengue shock and/or organ failure. A combination of PCT ≥0.7 ng/mL and PVL ≥2.5 mmol/L provided good prognostic value for predicting dengue shock and/or organ failure, with an area under the receiver operating characteristics curve of 0.83 (95% CI: 0.74-0.92), a sensitivity of 81.2% (95% CI: 63.6-92.8%), and a specificity of 84.4% (95% CI: 76.9-90.2%). Dengue shock patients with non-clearance of PCT and PVL expired during hospitalization.

Conclusions/significance: PCT ≥0.7 ng/mL and PVL ≥2.5 mmol/L were independently associated with dengue shock and/or organ failure. The combination of PCT and PVL levels could be used as prognostic biomarkers for the prediction of dengue shock and/or organ failure.

Publication types

  • Observational Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Biomarkers / blood*
  • Calcitonin / blood*
  • Dengue Virus / isolation & purification
  • Female
  • Hospitalization
  • Humans
  • Lactic Acid / blood*
  • Male
  • Middle Aged
  • Multiple Organ Failure / diagnosis*
  • Multiple Organ Failure / epidemiology
  • Multiple Organ Failure / virology
  • Odds Ratio
  • Prognosis
  • Prospective Studies
  • RNA, Viral / blood
  • ROC Curve
  • Risk Factors
  • Sensitivity and Specificity
  • Severe Dengue / blood
  • Severe Dengue / diagnosis*
  • Severe Dengue / epidemiology
  • Severe Dengue / virology
  • Thailand / epidemiology
  • Young Adult

Substances

  • Biomarkers
  • RNA, Viral
  • Lactic Acid
  • Calcitonin

Grants and funding

This study was supported by the Dean's Fund Research 2013, Faculty of Tropical Medicine, Mahidol University; Research Grant 2013, the Royal College of Physicians of Thailand; and Roche Diagnostics (Thailand) Ltd. for providing Elecsys BRAHMS PCT for this study. The funders had no role in study design, data collection and/or analysis, the decision to publish, or preparation of the manuscript.