Vitamin B1-deficient mice show impairment of hippocampus-dependent memory formation and loss of hippocampal neurons and dendritic spines: potential microendophenotypes of Wernicke-Korsakoff syndrome

Biosci Biotechnol Biochem. 2016 Dec;80(12):2425-2436. doi: 10.1080/09168451.2016.1224639. Epub 2016 Aug 31.

Abstract

Patients with severe Wernicke-Korsakoff syndrome (WKS) associated with vitamin B1 (thiamine) deficiency (TD) show enduring impairment of memory formation. The mechanisms of memory impairment induced by TD remain unknown. Here, we show that hippocampal degeneration is a potential microendophenotype (an endophenotype of brain disease at the cellular and synaptic levels) of WKS in pyrithiamine-induced thiamine deficiency (PTD) mice, a rodent model of WKS. PTD mice show deficits in the hippocampus-dependent memory formation, although they show normal hippocampus-independent memory. Similarly with WKS, impairments in memory formation did not recover even at 6 months after treatment with PTD. Importantly, PTD mice exhibit a decrease in neurons in the CA1, CA3, and dentate gyrus (DG) regions of the hippocampus and reduced density of wide dendritic spines in the DG. Our findings suggest that TD induces hippocampal degeneration, including the loss of neurons and spines, thereby leading to enduring impairment of hippocampus-dependent memory formation.

Keywords: Wernicke–Korsakoff syndrome; hippocampus-dependent memory; microendophenotypes; thiamine deficiency; vitamin B1.

MeSH terms

  • Amygdala / drug effects
  • Amygdala / physiopathology
  • Animals
  • Ataxia / complications
  • Body Weight
  • Dendritic Spines / pathology*
  • Hippocampus / pathology
  • Hippocampus / physiopathology*
  • Korsakoff Syndrome*
  • Memory*
  • Mice
  • Phenotype*
  • Pyrithiamine / pharmacology
  • Thiamine Deficiency / chemically induced
  • Thiamine Deficiency / complications
  • Thiamine Deficiency / pathology*
  • Thiamine Deficiency / physiopathology*

Substances

  • Pyrithiamine