Anti-encystment and amoebicidal activity of Lonicera japonica Thunb. and its major constituent chlorogenic acid in vitro

Tooba Mahboob; Abdul-Majid Azlan; Tian-Chye Tan; Chandramathi Samudi; Shamala Devi Sekaran; Veeranoot Nissapatorn; Christophe Wiart

doi:10.1016/j.apjtm.2016.07.008

Anti-encystment and amoebicidal activity of Lonicera japonica Thunb. and its major constituent chlorogenic acid in vitro

Asian Pac J Trop Med. 2016 Sep;9(9):866-871. doi: 10.1016/j.apjtm.2016.07.008. Epub 2016 Jul 26.

Authors

Tooba Mahboob¹, Abdul-Majid Azlan¹, Tian-Chye Tan¹, Chandramathi Samudi², Shamala Devi Sekaran², Veeranoot Nissapatorn³, Christophe Wiart⁴

Affiliations

¹ Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
² Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
³ Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: veeranoot@um.edu.my.
⁴ School of Pharmacy, University of Nottingham Malaysia Campus, Jalan 56000 Semenyih, Kuala Lumpur, Malaysia.

PMID: 27633300
DOI: 10.1016/j.apjtm.2016.07.008

Abstract

Objective: To examine the acanthamoebicidal effects of ethyl acetate, aqueous and butanol fractions of dried flower buds of Lonicera japonica (L. japonica) Thunb. (Flos Lonicerae) in vitro.

Methods: Acanthamoeba triangularis isolates were obtained from environmental water samples and identified by PCR. They were exposed to ethyl acetate, water and butanol fractions of L. japonica Thunb. at concentrations ranging from 0.5 mg/mL to 1.5 mg/mL. The extracts were evaluated for growth inhibition at 24, 48 and 72 h, respectively. Chlorogenic acid at a concentration of 1 mg/mL was examined for inhibition of encystment.

Results: Ethyl acetate fraction at a concentration of 1.5 mg/mL evoked a significant reduction of trophozoite viability by 48.9% after 24 h, 49.2% after 48 h and 33.7% after 72 h chlorogenic acid, the major active constituent of L. japonica Thunb. at the concentration of 1 mg/mL reduced the cysts/trophozoite ratio by 100% after 24 h, 84.0% after 48 h and 72.3% after 72 h. This phenolic compound at concentration of 1 mg/mL concurrent with 0.6% hydrogen peroxide inhibited hydrogen peroxide-induced encystment by 92.8% at 72 h.

Conclusions: Results obtained from this study show that ethyl acetate fraction at 1.5 mg/mL is the most potent fraction of L. japonica Thunb. and its major constituent chlorogenic acid showed the remarkable inhibition of encystment at a concentration of 1 mg/mL.

Keywords: Acanthamoeba spp.; Amoebicidal; Chlorogenic acid; Encystment; Lonicera japonica Thunb..