Decreased expression of IDH1-R132H correlates with poor survival in gastrointestinal cancer

Oncotarget. 2016 Nov 8;7(45):73638-73650. doi: 10.18632/oncotarget.12039.

Abstract

Isocitrate dehydrogenase (IDH1) is an NADP-dependent enzyme that catalyzes the decarboxylation of isocitrate to alpha-ketoglutarate. The IDH1-R132H mutation predicts a better clinical outcome for glioma patients, and the expression of IDH1-R132H correlates with a favorable outcome in patients with brain tumors. Here, we investigated IDH1-R132H expression in both gastric (n=526) and colorectal (n=399) tissues by performing immunohistochemistry analyses on tissue microarrays. We also tested whether IDH1-R132H expression correlated with various clinical parameters. In both gastric and colorectal cancer, expression of IDH1-R132H was associated with tumor stage. Patients with low IDH1-R132H expression had a poor overall survival. Our data indicate that IDH1-R132H expression could be used as a predictive marker of prognosis for patients with gastrointestinal cancer.

Keywords: IDH1-R132H; gastrointestinal cancer; immunohistochemistry; prognosis; tissue microarray.

MeSH terms

  • Adult
  • Aged
  • Amino Acid Substitution
  • Biomarkers, Tumor
  • Cell Line, Tumor
  • Codon
  • Female
  • Gastrointestinal Neoplasms / genetics*
  • Gastrointestinal Neoplasms / metabolism
  • Gastrointestinal Neoplasms / mortality*
  • Gastrointestinal Neoplasms / pathology
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • Isocitrate Dehydrogenase / genetics*
  • Isocitrate Dehydrogenase / metabolism
  • Kaplan-Meier Estimate
  • Male
  • Middle Aged
  • Mutation*
  • Neoplasm Grading
  • Neoplasm Staging
  • Prognosis

Substances

  • Biomarkers, Tumor
  • Codon
  • Isocitrate Dehydrogenase
  • IDH1 protein, human