A Glo1-Methylglyoxal Pathway that Is Perturbed in Maternal Diabetes Regulates Embryonic and Adult Neural Stem Cell Pools in Murine Offspring

Cell Rep. 2016 Oct 18;17(4):1022-1036. doi: 10.1016/j.celrep.2016.09.067.

Abstract

Maternal diabetes is known to adversely influence brain development in offspring. Here, we provide evidence that this involves the circulating metabolite methylglyoxal, which is increased in diabetes, and its detoxifying enzyme, glyoxalase 1 (Glo1), which when mutated is associated with neurodevelopmental disorders. Specifically, when Glo1 levels were decreased in embryonic mouse cortical neural precursor cells (NPCs), this led to premature neurogenesis and NPC depletion embryonically and long-term alterations in cortical neurons postnatally. Increased circulating maternal methylglyoxal caused similar changes in embryonic cortical precursors and neurons and long-lasting changes in cortical neurons and NPCs in adult offspring. Depletion of embryonic and adult NPCs was also observed in murine offspring exposed to a maternal diabetic environment. Thus, the Glo1-methylglyoxal pathway integrates maternal and NPC metabolism to regulate neural development, and perturbations in this pathway lead to long-lasting alterations in adult neurons and NPC pools.

Keywords: Glyoxalase 1; autism spectrum disorder; cortical development; maternal diabetes; methylglyoxal; neural stem cells; neurodevelopmental disorders; neurogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult Stem Cells / metabolism*
  • Animals
  • Animals, Newborn
  • Behavior, Animal
  • Cerebral Cortex / cytology
  • Cerebral Cortex / embryology
  • Diabetes Mellitus, Experimental / metabolism*
  • Diabetes Mellitus, Experimental / pathology
  • Diabetes, Gestational / metabolism
  • Diabetes, Gestational / pathology
  • Female
  • Gene Knockdown Techniques
  • HEK293 Cells
  • Humans
  • Lactoylglutathione Lyase / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Mouse Embryonic Stem Cells / metabolism*
  • Neural Stem Cells / metabolism*
  • Neurogenesis
  • Neurons / cytology
  • Neurons / metabolism
  • Pregnancy
  • Pyruvaldehyde / metabolism*
  • Signal Transduction*

Substances

  • Pyruvaldehyde
  • Glo1 protein, mouse
  • Lactoylglutathione Lyase