A Novel Blood Viscosity Estimation Method Based on Pressure-Flow Characteristics of an Oxygenator During Cardiopulmonary Bypass

Artif Organs. 2017 Mar;41(3):262-266. doi: 10.1111/aor.12747. Epub 2016 Oct 26.

Abstract

During cardiopulmonary bypass (CPB), blood viscosity conspicuously increases and decreases due to changes in hematocrit and blood temperature. Nevertheless, blood viscosity is typically not evaluated, because there is no technology that can provide simple, continuous, noncontact monitoring. We modeled the pressure-flow characteristics of an oxygenator in a previous study, and in that study we quantified the influence of viscosity on oxygenator function. The pressure-flow monitoring information in the oxygenator is derived from our model and enables the estimation of viscosity. The viscosity estimation method was proposed and investigated in an in vitro experiment. Three samples of whole bovine blood with different hematocrit levels (21.8, 31.0, and 39.8%) were prepared and perfused into the oxygenator. As the temperature changed from 37°C to 27°C, the mean inlet pressure (Pin ) and outlet pressure (Pout ) of the oxygenator and the flow (Q) and viscosity of the blood were measured. The estimated viscosity was calculated from the pressure gradient (ΔP = Pin - Pout ) and Q and was compared to the measured blood viscosity. A strong correlation was found between the two methods for all samples. Bland-Altman analysis revealed a mean bias of -0.0263 mPa.s, a standard deviation of 0.071 mPa.s, limits of agreement of -0.114-0.166 mPa.s, and a percent error of 5%. Therefore, this method is considered compatible with the torsional oscillation viscometer that has plus or minus 5% measurement accuracy. Our study offers the possibility of continuously estimating blood viscosity during CPB.

Keywords: Blood viscosity; Cardiopulmonary bypass; Oxygenator; Pressure gradient.

MeSH terms

  • Animals
  • Blood Flow Velocity
  • Blood Pressure*
  • Blood Viscosity*
  • Cardiopulmonary Bypass / instrumentation*
  • Cattle
  • Hematocrit
  • Models, Cardiovascular*
  • Oxygenators*
  • Predictive Value of Tests
  • Reproducibility of Results
  • Temperature
  • Time Factors