Isolation of Coronavirus NL63 from Blood from Children in Rural Haiti: Phylogenetic Similarities with Recent Isolates from Malaysia

Am J Trop Med Hyg. 2017 Jan 11;96(1):144-147. doi: 10.4269/ajtmh.16-0585. Epub 2016 Oct 31.

Abstract

Human coronavirus (HCoV) NL63 is recognized as a common cause of upper respiratory infections and influenza-like illness. In screening children with acute undifferentiated febrile illness in a school cohort in rural Haiti, we identified HCoV-NL63 in blood samples from four children. Cases clustered over an 11-day period; children did not have respiratory symptoms, but two had gastrointestinal complaints. On phylogenetic analysis, the Haitian HCoV-NL63 strains cluster together in a highly supported monophyletic clade linked most closely with recently reported strains from Malaysia; two respiratory HCoV-NL63 strains identified in north Florida in the same general period form a separate clade, albeit again with close linkages with the Malaysian strains. Our data highlight the variety of presentations that may be seen with HCoV-NL63, and underscore the apparent ease with which CoV strains move among countries, with our data consistent with recurrent introduction of strains into the Caribbean (Haiti and Florida) from Asia.

MeSH terms

  • Cell Line
  • Child
  • Coronavirus / classification*
  • Coronavirus / genetics
  • Coronavirus Infections / epidemiology*
  • Coronavirus Infections / virology*
  • Female
  • Genotype
  • Haiti / epidemiology
  • Humans
  • Malaysia / epidemiology
  • Male
  • Phylogeny
  • Rural Population