Lipid Membrane Encapsulation of a 3D DNA Nano Octahedron

Steven D Perrault; William M Shih

doi:10.1007/978-1-4939-6454-3_12

Lipid Membrane Encapsulation of a 3D DNA Nano Octahedron

Methods Mol Biol. 2017:1500:165-184. doi: 10.1007/978-1-4939-6454-3_12.

Authors

Steven D Perrault^{1

2

3}, William M Shih^{4

5

6}

Affiliations

¹ Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, CLSB 5th Floor, Blackfan Circle, Boston, MA, 02115, USA. Steven.Perrault@Wyss.Harvard.Edu.
² Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, 02115, USA. Steven.Perrault@Wyss.Harvard.Edu.
³ Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA, 02115, USA. Steven.Perrault@Wyss.Harvard.Edu.
⁴ Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, CLSB 5th Floor, Blackfan Circle, Boston, MA, 02115, USA.
⁵ Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, 02115, USA.
⁶ Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA, 02115, USA.

PMID: 27813008
DOI: 10.1007/978-1-4939-6454-3_12

Abstract

Structural DNA nanotechnology methods such as DNA origami allow for the synthesis of highly precise nanometer-scale materials (Rothemund, Nature 440:297-302, 2006; Douglas et al., Nature 459:414-418, 2009). These offer compelling advantages for biomedical applications. Such materials can suffer from structural instability in biological environments due to denaturation and nuclease digestion (Hahn et al., ACS Nano 2014; Perrault and Shih, ACS Nano 8:5132-5140, 2014). Encapsulation of DNA nanostructures in a lipid membrane compartmentalizes them from their environment and prevents denaturation and nuclease digestion (Perrault and Shih, ACS Nano 8:5132-5140, 2014). Here, we describe the encapsulation of a 50 nm DNA nanostructure having the geometry of a wireframe octahedron in a phospholipid membrane containing poly-(ethylene glycol), resulting in biocompatible DNA nanostructures.

Keywords: DNA; Liposome; NanoOctahedron; Nanotechnology; Vesicle.

MeSH terms

DNA / chemistry*
Membrane Lipids / chemistry*
Membranes / chemistry*
Nanostructures / chemistry*
Nanotechnology / methods
Polyethylene Glycols / chemistry

Substances

Membrane Lipids
Polyethylene Glycols
DNA