Hyperferritinemia is a potential marker of chronic chikungunya: A retrospective study on the Island of Curaçao during the 2014-2015 outbreak

J Clin Virol. 2017 Jan:86:31-38. doi: 10.1016/j.jcv.2016.11.003. Epub 2016 Nov 18.

Abstract

Background: Recently Chikungunya virus (CHIKV) outbreaks have been reported in the Carribean. There is no data regarding the outbreak in Curaçao. In addition, to date there is no biomarker that could be used to predict chronic infection.

Objectives: To characterize the first CHIKV outbreak in Curaçao and to identify potential biomarkers for chronic infection.

Study design: A serological test and quantitative polymerase chain reaction (qPCR) were used on samples collected in Curaçao to confirm infection. Subsequently, six samples with high viral load were selected for phylogenetic analysis. Furthermore we investigated the association of macrophage-related biomarkers during CHIKV infection with chronic arthralgia/arthritis.

Results: 116 patients in Curacao were diagnosed with CHIKV infection based on ELISA and 77% were tested positive for CHIKV by qPCR. Phylogenetic analysis showed that an Asian genotype was the cause of the outbreak. Elevated levels of ferritin and CRP were significantly associated with viraemia. In addition, elevated ferritin levels were significantly associated with chronic arthralgia.

Conclusions: The results showed that the presence of an Asian genotype of CHIKV in Curaçao for the first time. Moreover, we found an association between ferritin levels with chronic arthralgia.

Keywords: Chikungunya virus; Chronic arthralgia; Curaçao; Ferritin; Neopterin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral / blood
  • Biomarkers / blood*
  • Chikungunya Fever / pathology*
  • Chikungunya virus / classification
  • Chikungunya virus / genetics
  • Chikungunya virus / isolation & purification
  • Chronic Disease / epidemiology*
  • Curacao / epidemiology
  • Disease Outbreaks*
  • Enzyme-Linked Immunosorbent Assay
  • Ferritins / blood*
  • Genotype
  • Humans
  • Retrospective Studies
  • Viral Load

Substances

  • Antibodies, Viral
  • Biomarkers
  • Ferritins