Comparison of the transcriptomic profile of hepatic human induced pluripotent stem like cells cultured in plates and in a 3D microscale dynamic environment

Eric Leclerc; Keiichi Kimura; Marie Shinohara; Mathieu Danoy; Morgane Le Gall; Taketomo Kido; Atsushi Miyajima; Teruo Fujii; Yasuyuki Sakai

doi:10.1016/j.ygeno.2016.11.008

Comparison of the transcriptomic profile of hepatic human induced pluripotent stem like cells cultured in plates and in a 3D microscale dynamic environment

Genomics. 2017 Jan;109(1):16-26. doi: 10.1016/j.ygeno.2016.11.008. Epub 2016 Nov 29.

Authors

Eric Leclerc¹, Keiichi Kimura², Marie Shinohara², Mathieu Danoy³, Morgane Le Gall⁴, Taketomo Kido⁵, Atsushi Miyajima⁵, Teruo Fujii⁶, Yasuyuki Sakai⁶

Affiliations

¹ CNRS UMI 2820, Laboratory for Integrated Micro Mechatronic Systems, Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. Electronic address: eric.leclerc@utc.fr.
² CIBIS, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
³ CNRS UMI 2820, Laboratory for Integrated Micro Mechatronic Systems, Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; IEMN CNRS UMR 8520, 59652 Villeneuve d'Ascq, France.
⁴ Inserm U1016 Institut Cochin, CNRS UMR8104, Plateforme Protéomique 3P5 Université Paris Descartes, Sorbonne Paris Cité, 22 rue Mechain, Paris 75014, France.
⁵ Laboratory of Cell Growth and Differentiation, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
⁶ CIBIS, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; CNRS UMI 2820, Laboratory for Integrated Micro Mechatronic Systems, Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.

PMID: 27913249
DOI: 10.1016/j.ygeno.2016.11.008

Abstract

We have compared the transcriptomic profiles of human induced pluripotent stem cells after their differentiation in hepatocytes like cells in plates and microfluidic biochips. The biochips provided a 3D and dynamic support during the cell differentiation when compared to the 2D static cultures in plates. The microarray have demonstrated the up regulation of important pathway related to liver development and maturation during the culture in biochips. Furthermore, the results of the transcriptomic profile, coupled with immunostaining, and RTqPCR analysis have shown typical biomarkers illustrating the presence of responders of biliary like cells, hepatocytes like cells, and endothelial like cells. However, the overall tissue still presented characteristic of immature and foetal patterns. Nevertheless, the biochip culture provided a specific micro-environment in which a complex multicellular differentiation toward liver could be oriented.

Keywords: Bioreactor; Hepatocytes; Human induced pluripotent stem cells; Liver.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bioreactors
Cell Differentiation*
Cells, Cultured
Humans
Induced Pluripotent Stem Cells / physiology*
Liver / cytology*
Liver / physiology
Microarray Analysis
Transcriptome*