Identification of a CD133-CD55- population functions as a fetal common skeletal progenitor

Lihong Weng; Xingbin Hu; Bijender Kumar; Mayra Garcia; Ivan Todorov; Xiaoman Jung; Guido Marcucci; Stephen J Forman; Ching-Cheng Chen

doi:10.1038/srep38632

Identification of a CD133-CD55- population functions as a fetal common skeletal progenitor

Sci Rep. 2016 Dec 8:6:38632. doi: 10.1038/srep38632.

Authors

Lihong Weng^{1

2

3}, Xingbin Hu^{1

4}, Bijender Kumar^{1

2}, Mayra Garcia^{1

2}, Ivan Todorov⁵, Xiaoman Jung¹, Guido Marcucci^{1

2}, Stephen J Forman^{2

3

6}, Ching-Cheng Chen^{1

2

6}

Affiliations

¹ Divison of Hematopoietic Stem Cell and Leukemia Research, Gehr Family Center for Leukemia Research, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.
² Department of Hematology and Hematopoietic Cell Transplantation, City of Hope, Duarte, CA 91010, USA.
³ Departments of Cancer Immunotherapeutic and Immunology, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.
⁴ Department of Transfusion Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 7100032, P.R. China.
⁵ Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.
⁶ Irell &Manella Graduate School of Biological Sciences, City of Hope, Duarte, CA 91010, USA.

Abstract

In this study, we identified a CD105+CD90.1-CD133-CD55- (CD133-CD55-) population in the fetal skeletal element that can generate bone and bone marrow. Besides osteoblasts and chondrocytes, the CD133-CD55- common progenitors can give rise to marrow reticular stromal cells and perivascular mesenchymal progenitors suggesting they function as the fetal common skeletal progenitor. Suppression of CXCL12 and Kitl expression in CD133-CD55- common progenitors severely disrupted the BM niche formation but not bone generation. Thus, CD133-CD55- common progenitors are the main source of CXCL12 and Kitl producing cells in the developing marrow.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

AC133 Antigen / metabolism*
Animals
Ataxin-1 / metabolism
Biomarkers
Bone Marrow / metabolism
CD55 Antigens / metabolism*
Cell Differentiation
Chemokine CXCL12 / metabolism
Chondrocytes / metabolism
Colony-Forming Units Assay
Immunophenotyping
Mice
Mice, Knockout
Osteoblasts / cytology
Osteoblasts / metabolism*
Osteogenesis
Phenotype
Stem Cell Niche

Substances

AC133 Antigen
Ataxin-1
Biomarkers
CD55 Antigens
Chemokine CXCL12

Abstract

Publication types

MeSH terms

Substances

Grants and funding