Whole-Organism Developmental Expression Profiling Identifies RAB-28 as a Novel Ciliary GTPase Associated with the BBSome and Intraflagellar Transport

PLoS Genet. 2016 Dec 8;12(12):e1006469. doi: 10.1371/journal.pgen.1006469. eCollection 2016 Dec.

Abstract

Primary cilia are specialised sensory and developmental signalling devices extending from the surface of most eukaryotic cells. Defects in these organelles cause inherited human disorders (ciliopathies) such as retinitis pigmentosa and Bardet-Biedl syndrome (BBS), frequently affecting many physiological and developmental processes across multiple organs. Cilium formation, maintenance and function depend on intracellular transport systems such as intraflagellar transport (IFT), which is driven by kinesin-2 and IFT-dynein motors and regulated by the Bardet-Biedl syndrome (BBS) cargo-adaptor protein complex, or BBSome. To identify new cilium-associated genes, we employed the nematode C. elegans, where ciliogenesis occurs within a short timespan during late embryogenesis when most sensory neurons differentiate. Using whole-organism RNA-Seq libraries, we discovered a signature expression profile highly enriched for transcripts of known ciliary proteins, including FAM-161 (FAM161A orthologue), CCDC-104 (CCDC104), and RPI-1 (RP1/RP1L1), which we confirm are cilium-localised in worms. From a list of 185 candidate ciliary genes, we uncover orthologues of human MAP9, YAP, CCDC149, and RAB28 as conserved cilium-associated components. Further analyses of C. elegans RAB-28, recently associated with autosomal-recessive cone-rod dystrophy, reveal that this small GTPase is exclusively expressed in ciliated neurons where it dynamically associates with IFT trains. Whereas inactive GDP-bound RAB-28 displays no IFT movement and diffuse localisation, GTP-bound (activated) RAB-28 concentrates at the periciliary membrane in a BBSome-dependent manner and undergoes bidirectional IFT. Functional analyses reveal that whilst cilium structure, sensory function and IFT are seemingly normal in a rab-28 null allele, overexpression of predicted GDP or GTP locked variants of RAB-28 perturbs cilium and sensory pore morphogenesis and function. Collectively, our findings present a new approach for identifying ciliary proteins, and unveil RAB28, a GTPase most closely related to the BBS protein RABL4/IFT27, as an IFT-associated cargo with BBSome-dependent cell autonomous and non-autonomous functions at the ciliary base.

MeSH terms

  • Animals
  • Bardet-Biedl Syndrome / genetics
  • Bardet-Biedl Syndrome / pathology
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / growth & development
  • Caenorhabditis elegans Proteins / genetics*
  • Cell Membrane / genetics
  • Cilia / genetics*
  • Cilia / metabolism
  • Dendrites / genetics
  • Dyneins / biosynthesis
  • Dyneins / genetics
  • Embryonic Development / genetics*
  • Flagella / genetics
  • GTP Phosphohydrolases / genetics*
  • Gene Expression Regulation, Developmental
  • Humans
  • Kinesins / biosynthesis
  • Kinesins / genetics
  • Protein Transport / genetics
  • Retinitis Pigmentosa / genetics
  • Retinitis Pigmentosa / pathology
  • Sensory Receptor Cells / metabolism
  • rab GTP-Binding Proteins / biosynthesis*
  • rab GTP-Binding Proteins / genetics

Substances

  • Caenorhabditis elegans Proteins
  • KIF2A protein, human
  • GTP Phosphohydrolases
  • Dyneins
  • Kinesins
  • Rab-28 protein, C elegans
  • rab GTP-Binding Proteins

Grants and funding

MRL acknowledges funding from the Canadian Institutes of Health Research (CIHR; grant MOP-82870) and a senior scholarship award from Michael Smith Foundation for Health Research (MSFHR). RDM acknowledges funding from the Natural Sciences and Engineering Council of Canada (NSERC; 435398-2013) and holds a CIHR New Investigator Award. VLJ holds postdoctoral fellowships from MSFHR and KRESCENT, and TAT is the recipient of a Banting Postdoctoral Fellowship. OEB acknowledges principal investigator funding from Science Foundation Ireland (11/PI/1037), and SC holds an Irish Research Council Government of Ireland postgraduate award (GOIPG/2014/683). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.