High Mass Ion Detection with Charge Detector Coupled to Rectilinear Ion Trap Mass Spectrometer

Avinash A Patil; Szu-Wei Chou; Pei-Yu Chang; Chen-Wei Lee; Chun-Yen Cheng; Ming-Lee Chu; Wen-Ping Peng

doi:10.1007/s13361-016-1548-0

High Mass Ion Detection with Charge Detector Coupled to Rectilinear Ion Trap Mass Spectrometer

J Am Soc Mass Spectrom. 2017 Jun;28(6):1066-1078. doi: 10.1007/s13361-016-1548-0. Epub 2016 Dec 13.

Authors

Avinash A Patil¹, Szu-Wei Chou^{1

2}, Pei-Yu Chang¹, Chen-Wei Lee¹, Chun-Yen Cheng², Ming-Lee Chu³, Wen-Ping Peng⁴

Affiliations

¹ Department of Physics, National Dong Hwa University, Shoufeng, Hualien, Taiwan, 97401, Republic of China.
² AcroMass technologies Inc., Hukou, Hsinchu, Taiwan, 30352, Republic of China.
³ Institute of Physics, Academia Sinica, Taipei, Taiwan, Republic of China.
⁴ Department of Physics, National Dong Hwa University, Shoufeng, Hualien, Taiwan, 97401, Republic of China. pengw@gms.ndhu.edu.tw.

PMID: 27966174
DOI: 10.1007/s13361-016-1548-0

Abstract

Conventional linear ion trap mass analyzers (LIT-MS) provide high ion capacity and show their MS ⁿ ability; however, the detection of high mass ions is still challenging because LIT-MS with secondary electron detectors (SED) cannot detect high mass ions. To detect high mass ions, we coupled a charge detector (CD) to a rectilinear ion trap mass spectrometer (RIT-MS). Immunoglobulin G ions (m/z ~150,000) are measured successfully with controlled ion kinetic energy. In addition, when mass-to-charge (m/z) ratios of singly charged ions exceed 10 kTh, the detection efficiency of CD is found to be greater than that of SED. The CD can be coupled to LIT-MS to extend the detection mass range and provide the potential to perform MS ⁿ of high mass ions inside the ion trap. Graphical Abstract ᅟ.

Keywords: Buffer gas pressure; Charge detector; High mass proteins; LDI/MALDI ions; Noise rejection; Orthogonal wavelet packet decomposition; Quadrupole ion guide; Scan rate; Voltage-scan rectilinear ion trap mass analyzer.