Metabolomics study of Populus type propolis

Boban Anđelković; Ljubodrag Vujisić; Ivan Vučković; Vele Tešević; Vlatka Vajs; Dejan Gođevac

doi:10.1016/j.jpba.2016.12.003

Metabolomics study of Populus type propolis

J Pharm Biomed Anal. 2017 Feb 20:135:217-226. doi: 10.1016/j.jpba.2016.12.003. Epub 2016 Dec 15.

Authors

Boban Anđelković¹, Ljubodrag Vujisić¹, Ivan Vučković¹, Vele Tešević¹, Vlatka Vajs², Dejan Gođevac³

Affiliations

¹ Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
² Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
³ Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia. Electronic address: dgodjev@chem.bg.ac.rs.

PMID: 28012592
DOI: 10.1016/j.jpba.2016.12.003

Abstract

Herein, we propose rapid and simple spectroscopic methods to determine the chemical composition of propolis derived from various Populus species using a metabolomics approach. In order to correlate variability in Populus type propolis composition with the altitude of its collection, NMR, IR, and UV spectroscopy followed by OPLS was conducted. The botanical origin of propolis was established by comparing propolis spectral data to those of buds of various Populus species. An O2PLS method was utilized to integrate two blocks of data. According to OPLS and O2PLS, the major compounds in propolis samples, collected from temperate continental climate above 500m, were phenolic glycerides originating from P. tremula buds. Flavonoids were predominant in propolis samples collected below 400m, originating from P. nigra and P. x euramericana buds. Samples collected at 400-500m were of mixed origin, with variable amounts of all detected metabolites.

Keywords: IR; NMR; O2PLS; OPLS; Propolis; UV.

MeSH terms

Animals
Magnetic Resonance Spectroscopy / methods*
Metabolomics / methods*
Multivariate Analysis
Populus / chemistry
Populus / metabolism*
Propolis / analysis
Propolis / metabolism*

Substances

Propolis