Activating mutations and translocations in the guanine exchange factor VAV1 in peripheral T-cell lymphomas

Proc Natl Acad Sci U S A. 2017 Jan 24;114(4):764-769. doi: 10.1073/pnas.1608839114. Epub 2017 Jan 6.

Abstract

Peripheral T-cell lymphomas (PTCLs) are a heterogeneous group of non-Hodgkin lymphomas frequently associated with poor prognosis and for which genetic mechanisms of transformation remain incompletely understood. Using RNA sequencing and targeted sequencing, here we identify a recurrent in-frame deletion (VAV1 Δ778-786) generated by a focal deletion-driven alternative splicing mechanism as well as novel VAV1 gene fusions (VAV1-THAP4, VAV1-MYO1F, and VAV1-S100A7) in PTCL. Mechanistically these genetic lesions result in increased activation of VAV1 catalytic-dependent (MAPK, JNK) and non-catalytic-dependent (nuclear factor of activated T cells, NFAT) VAV1 effector pathways. These results support a driver oncogenic role for VAV1 signaling in the pathogenesis of PTCL.

Keywords: VAV1; gene fusion; mutation; peripheral T-cell lymphoma.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing / genetics
  • Amino Acid Sequence
  • Base Sequence
  • Cell Line, Tumor
  • Guanine / metabolism*
  • Guanine Nucleotide Exchange Factors / genetics*
  • Humans
  • Jurkat Cells
  • Lymphoma, T-Cell, Peripheral / genetics*
  • Mutation / genetics*
  • Proto-Oncogene Proteins c-vav / genetics*
  • Sequence Deletion / genetics
  • Translocation, Genetic / genetics*

Substances

  • Guanine Nucleotide Exchange Factors
  • Proto-Oncogene Proteins c-vav
  • VAV1 protein, human
  • Guanine