Association of MMP-2 and MMP-9 expression with recurrences in primary spontaneous pneumothorax

Kaohsiung J Med Sci. 2017 Jan;33(1):17-23. doi: 10.1016/j.kjms.2016.10.007. Epub 2016 Nov 12.

Abstract

Primary spontaneous pneumothorax (PSP) is a common benign problem. However, PSP recurrence is still a troublesome complication for most patients. This study intended to determine the role of matrix metalloproteinase-2 (MMP-2) and MMP-9 in type II pneumocytes of patients with PSP and its relation with recurrence. Ninety-one patients who had undergone needlescopic video-assisted thoracoscopic surgery wedge resection of lung with identifiable blebs for PSP were included in this study. Immunohistochemical (IHC) staining was used to measure the expression of MMP-2 and MMP-9 in lung tissues of PSP patients. The results were further correlated with clinicopathological parameters and recurrence rates using chi-square or Fisher's exact test. The value of MMP-2 and MMP-9 for overall recurrence was analyzed by univariate and multivariable Cox regression model. IHC data revealed that MMP-2 and MMP-9 staining was predominantly observed in type II pneumocytes of patients with PSP. We found that MMP-2 and MMP-9 expression in PSP, especially male PSP patients, was significantly correlated with recurrence. In the univariate and multivariate analyses, MMP-2 and MMP-9 were statistically significant risk factors for overall recurrence in PSP patients. Therefore, high expression levels of MMP-2 and MMP-9 in type II pneumocytes show a positive correlation with PSP recurrence risk. Further studies are needed to validate whether reduction of MMP-2 and MMP-9 expression may be a promising way for decreasing the risk of PSP recurrence in the future.

Keywords: Matrix metalloproteinase; Pneumothorax; Recurrence; Type II pneumocyte.

MeSH terms

  • Adolescent
  • Adult
  • Alveolar Epithelial Cells / enzymology*
  • Alveolar Epithelial Cells / pathology
  • Female
  • Gene Expression
  • Humans
  • Immunohistochemistry
  • Lung / diagnostic imaging
  • Lung / enzymology*
  • Lung / pathology
  • Lung / surgery
  • Male
  • Matrix Metalloproteinase 2 / genetics*
  • Matrix Metalloproteinase 2 / metabolism
  • Matrix Metalloproteinase 9 / genetics*
  • Matrix Metalloproteinase 9 / metabolism
  • Multivariate Analysis
  • Pneumothorax / diagnostic imaging
  • Pneumothorax / enzymology
  • Pneumothorax / genetics*
  • Pneumothorax / surgery
  • Radiography
  • Recurrence
  • Retrospective Studies
  • Risk Factors
  • Thoracic Surgery, Video-Assisted*

Substances

  • MMP2 protein, human
  • Matrix Metalloproteinase 2
  • MMP9 protein, human
  • Matrix Metalloproteinase 9