Abstract
We recently identified PHF13 as an H3K4me2/3 chromatin reader and transcriptional co-regulator. We found that PHF13 interacts with RNAPIIS5P and PRC2 stabilizing their association with active and bivalent promoters. Furthermore, mass spectrometry analysis identified ∼50 spliceosomal proteins in PHF13s interactome. Here, we will discuss the potential role of PHF13 in RNAPII pausing and co-transcriptional splicing.
Keywords:
PHF13; RNA polymerase II; RNA polymerase II pausing; SPOC1; bivalency and H3K4me3; co-transcriptional splicing; polycomb repressive complex 2 (PRC2); transcriptional regulation.
MeSH terms
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Animals
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Chromatin / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Histone Methyltransferases
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Histone-Lysine N-Methyltransferase / metabolism
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Histones / metabolism
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Humans
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Mice
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Polycomb-Group Proteins / genetics
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Polycomb-Group Proteins / metabolism
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Promoter Regions, Genetic
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RNA Polymerase II / genetics
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RNA Polymerase II / metabolism*
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RNA Splice Sites
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic / physiology*
Substances
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Chromatin
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DNA-Binding Proteins
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Histones
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PHF13 protein, human
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Polycomb-Group Proteins
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RNA Splice Sites
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Transcription Factors
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Histone Methyltransferases
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Histone-Lysine N-Methyltransferase
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RNA Polymerase II