Abstract
A CRISPR screen conducted in a CD4+ T cell leukemia line has identified host factors required for HIV infection but dispensable for cellular survival. The results highlight sulfation on the HIV co-receptor CCR5 and cellular aggregation as potential targets for therapeutic intervention.
MeSH terms
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Antigens, CD / genetics*
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Antigens, CD / immunology
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CD4 Antigens / genetics*
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CD4 Antigens / immunology
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / virology
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Cell Adhesion
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Cell Adhesion Molecules, Neuronal / genetics*
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Cell Adhesion Molecules, Neuronal / immunology
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Clustered Regularly Interspaced Short Palindromic Repeats
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Fetal Proteins / genetics*
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Fetal Proteins / immunology
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Gene Expression Regulation
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HIV Infections / immunology
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HIV Infections / pathology
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HIV Infections / virology
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HIV-1 / physiology*
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High-Throughput Screening Assays
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Host-Pathogen Interactions
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Humans
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Membrane Proteins / genetics*
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Membrane Proteins / immunology
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Membrane Transport Proteins / genetics*
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Membrane Transport Proteins / immunology
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Molecular Targeted Therapy
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Receptors, CCR5 / genetics*
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Receptors, CCR5 / immunology
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Sulfate Transporters
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Sulfotransferases / genetics*
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Sulfotransferases / immunology
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Virus Internalization
Substances
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ALCAM protein, human
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Antigens, CD
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CCR5 protein, human
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CD4 Antigens
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Cell Adhesion Molecules, Neuronal
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Fetal Proteins
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Membrane Proteins
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Membrane Transport Proteins
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Receptors, CCR5
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SLC35B2 protein, human
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Sulfate Transporters
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Sulfotransferases
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TPST2 protein, human