HOPX hypermethylation promotes metastasis via activating SNAIL transcription in nasopharyngeal carcinoma

Nat Commun. 2017 Feb 1:8:14053. doi: 10.1038/ncomms14053.

Abstract

Nasopharyngeal carcinoma (NPC) is characterized by a high rate of local invasion and early distant metastasis. Increasing evidence indicates that epigenetic abnormalities play important roles in NPC development. However, the epigenetic mechanisms underlying NPC metastasis remain unclear. Here we investigate aberrantly methylated transcription factors in NPC tissues, and we identify the HOP homeobox HOPX as the most significantly hypermethylated gene. Consistently, we find that HOXP expression is downregulated in NPC tissues and NPC cell lines. Restoring HOPX expression suppresses metastasis and enhances chemosensitivity of NPC cells. These effects are mediated by HOPX-mediated epigenetic silencing of SNAIL transcription through the enhancement of histone H3K9 deacetylation in the SNAIL promoter. Moreover, we find that patients with high methylation levels of HOPX exhibit poor clinical outcomes in both the training and validation cohorts. In summary, HOPX acts as a tumour suppressor via the epigenetic regulation of SNAIL transcription, which provides a novel prognostic biomarker for NPC metastasis and therapeutic target for NPC treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Animals
  • Cell Line, Tumor
  • DNA Methylation*
  • Female
  • Gene Expression Regulation, Neoplastic
  • Histones / metabolism
  • Homeodomain Proteins / genetics*
  • Humans
  • Kaplan-Meier Estimate
  • Mice, Inbred BALB C
  • Mice, Nude
  • Nasopharyngeal Neoplasms / genetics*
  • Nasopharyngeal Neoplasms / metabolism
  • Nasopharyngeal Neoplasms / pathology
  • Neoplasm Metastasis
  • Promoter Regions, Genetic / genetics
  • Snail Family Transcription Factors / genetics*
  • Transcriptional Activation*
  • Transplantation, Heterologous
  • Tumor Suppressor Proteins / genetics*

Substances

  • HOPX protein, human
  • Histones
  • Homeodomain Proteins
  • Snail Family Transcription Factors
  • Tumor Suppressor Proteins