Acute norovirus gastroenteritis in children in a highly rotavirus-vaccinated population in Northeast Brazil

J Clin Virol. 2017 Mar:88:33-38. doi: 10.1016/j.jcv.2016.10.015. Epub 2016 Oct 29.

Abstract

Background: Gastroenteritis is one of the most important causes of morbidity and mortality in children and an important etiological agent is norovirus.

Objective: We describe the occurrence and characteristics of norovirus diarrhoea in children from Sergipe, Northeast-Brazil, over two consecutive periods of three years following rotavirus vaccine introduction.

Study design: A cross sectional hospital-based survey conducted from October-2006 to September-2009 and from July-2011 to January-2013. Acute diarrhoea cases had a stool sample collected and tested for norovirus by RT-PCR and positive samples were sequenced.

Results: In total 280 (19.6%) of 1432 samples were norovirus positive, including 204 (18.3%) of 1113 samples collected during the first period and 76 (23.9%) of 318 collected during the second period. The proportion of children with norovirus infection increased significantly through the second study period (χ2 for trend=6.7; p=0.009), was more frequent in rotavirus vaccinated and in younger children (p<0.001). Of 280 norovirus-positive specimens, 188 (67.1%) were sequenced. Of these, 12 were genogroup I and 176 genogroup II. The main genotype was GII.4 (149/188, 79.3%), followed by GII.2 (6, 3.2%) and GII.6 (5, 2.6%).

Conclusion: Norovirus annual detection rates increased over the study period. The detection of norovirus was higher among young children.

Keywords: Brazil; Children; Diarrhoea; Norovirus; Rotavirus vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brazil
  • Caliciviridae Infections / epidemiology*
  • Child, Preschool
  • Cross-Sectional Studies
  • Diarrhea / epidemiology*
  • Diarrhea / virology
  • Feces / virology
  • Female
  • Gastroenteritis / epidemiology*
  • Gastroenteritis / virology
  • Genotype
  • Humans
  • Infant
  • Male
  • Norovirus / classification
  • Norovirus / genetics
  • Norovirus / isolation & purification*
  • Prevalence
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA