Measurement of Macrophage-Specific In Vivo Reverse Cholesterol Transport in Mice

Maaike Kockx; Wendy Jessup; Leonard Kritharides

doi:10.1007/978-1-4939-6875-6_21

Measurement of Macrophage-Specific In Vivo Reverse Cholesterol Transport in Mice

Methods Mol Biol. 2017:1583:287-298. doi: 10.1007/978-1-4939-6875-6_21.

Authors

Maaike Kockx¹, Wendy Jessup², Leonard Kritharides²

Affiliations

¹ ANZAC Research Institute, Concord Repatriation General Hospital, Concord, NSW, 2139, Australia. maaike.kockx@sydney.edu.au.
² ANZAC Research Institute, Concord Repatriation General Hospital, Concord, NSW, 2139, Australia.

PMID: 28205182
DOI: 10.1007/978-1-4939-6875-6_21

Abstract

Reverse cholesterol transport (RCT) is one of the main processes that is thought to protect against cardiovascular disease. RCT constitutes the removal of cholesterol from peripheral sites, its transport through the plasma compartment for delivery to the liver for excretion. Here, we describe an in vivo RCT method that incorporates these steps, measuring movement of cholesterol from macrophages to the plasma, the liver, and finally to the feces in mice.

Keywords: In vivo RCT; Macrophage-specific RCT; Reverse cholesterol transport.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biological Transport, Active / physiology
Cell Line
Cholesterol / metabolism*
Feces
Liver / metabolism
Macrophages / metabolism*
Mice

Substances

Cholesterol