Abstract
We report the molecular investigations of a large influenza A(H3N2) outbreak, in a season characterised by sharp increase in influenza admissions since December 2016. Analysis of haemagglutinin (HA) sequences demonstrated co-circulation of multiple clades (3C.3a, 3C.2a and 3C.2a1). Most variants fell into a novel subclade (proposed as 3C.2a2); they possessed four unique amino acid substitutions in the HA protein and loss of a potential glycosylation site. These changes potentially modify the H3N2 strain antigenicity.
Keywords:
influenza; molecular; next generation sequencing; outbreak.
This article is copyright of The Authors, 2017.
MeSH terms
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Amino Acid Substitution
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Child
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Child, Preschool
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Communicable Diseases, Emerging / epidemiology
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Communicable Diseases, Emerging / genetics*
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Epidemics*
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Female
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Genetic Drift
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Genetic Variation
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Glycosylation
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Hemagglutinin Glycoproteins, Influenza Virus / genetics*
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Hemagglutinins / genetics*
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Humans
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Infant
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Infant, Newborn
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Influenza A Virus, H3N2 Subtype / classification
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Influenza A Virus, H3N2 Subtype / genetics*
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Influenza A Virus, H3N2 Subtype / isolation & purification
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Influenza, Human / diagnosis
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Influenza, Human / epidemiology*
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Influenza, Human / virology
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London / epidemiology
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Male
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Middle Aged
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Molecular Sequence Data
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Phylogeny
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RNA, Viral / genetics
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RNA, Viral / isolation & purification
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Sequence Analysis, DNA
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Young Adult
Substances
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Hemagglutinin Glycoproteins, Influenza Virus
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Hemagglutinins
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RNA, Viral