Selenium nanoparticles decorated with Ulva lactuca polysaccharide potentially attenuate colitis by inhibiting NF-κB mediated hyper inflammation

J Nanobiotechnology. 2017 Mar 7;15(1):20. doi: 10.1186/s12951-017-0252-y.

Abstract

Background: Selenium (Se) is an essential micronutrient trace element and an established nutritional antioxidant. Low Se status exacerbates inflammatory bowel diseases progression, which involves hyper inflammation in the digestive tract. Se nanoparticles (SeNPs) exhibit anti-inflammatory activity accompanied by low toxicity, especially when decorated with natural biological compounds. Herein, we explored the beneficial effects of SeNPs decorated with Ulva lactuca polysaccharide (ULP) in mice subjected to the acute colitis model.

Results: We constructed SeNPs coated with ULP (ULP-SeNPs) in average diameter ~130 nm and demonstrated their stability and homogeneity. Supplementation with ULP-SeNPs (0.8 ppm Se) resulted in a significant protective effect on DSS-induced acute colitis in mice including mitigation of body weight loss, and colonic inflammatory damage. ULP-SeNPs ameliorated macrophage infiltration as evidenced by decreased CD68 levels in colon tissue sections. The anti-inflammatory effects of ULP-SeNPs were found to involve modulation of cytokines including IL-6 and TNF-α. Mechanistically, ULP-SeNPs inhibited the activation of macrophages by suppressing the nuclear translocation of NF-κB, which drives the transcription of these pro-inflammatory cytokines.

Conclusions: ULP-SeNPs supplementation may offer therapeutic potential for reducing the symptoms of acute colitis through its anti-inflammatory actions.

Keywords: Inflammatory bowel diseases (IBD); Nuclear factor κ-B (NF-κB); Selenium (Se); Selenium nanoparticles (SeNPs); Ulva lactuca polysaccharide (ULP).

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / therapeutic use*
  • Colitis / drug therapy*
  • Colitis / immunology
  • Colitis / pathology
  • Colon / drug effects*
  • Colon / immunology
  • Colon / pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • NF-kappa B / antagonists & inhibitors
  • NF-kappa B / immunology*
  • Nanoparticles / chemistry
  • Nanoparticles / therapeutic use*
  • Polysaccharides / chemistry
  • Polysaccharides / therapeutic use*
  • Selenium / chemistry
  • Selenium / therapeutic use*
  • Ulva / chemistry

Substances

  • Anti-Inflammatory Agents
  • NF-kappa B
  • Polysaccharides
  • Selenium