Interleukin 17A and Toll-like Receptor 4 in Patients with Arterial Hypertension

Kidney Blood Press Res. 2017;42(1):99-108. doi: 10.1159/000471900. Epub 2017 Apr 3.

Abstract

Background/aims: Immune responses are involved in arterial hypertension. An observational cross-sectional case control study was conducted to estimate the association between Toll-like receptor 4 (TLR4) expression and interleukin (IL)-17A serum levels in patients with controlled and non-controlled hypertension.

Methods: We have enrolled 105 non-complicated otherwise healthy hypertensive patients: 53 with well-controlled blood pressure and 52 non-controlled. TLR4 peripheral monocytes expression and serum IL-17A levels were determined by flow cytometry and ELISA, respectively.

Results: Non-controlled patients exhibited higher TLR4 expression than well-controlled (25.60 vs. 21.99, P=0.011). TLR4 expression was lower in well-controlled patients who were prescribed beta blockers (18.9 vs. 22.6, P=0.005) and IL-17A concentration was higher in patients using diuretics in either group (1.41 vs. 2.01 pg/ml, P<0.001; well-controlled 1.3 vs. 1.8 pg/ml, P= 0.023; non-controlled 1.6 vs. 2.3 pg/ml, P=0.001). Correlation between IL-17A concentration and hypertension duration was observed in non-controlled patients (Spearman correlation coefficient . ρ=0.566, P<0.001) whereas in well-controlled patients a correlation was found between hypertension duration and TLR4 expression (ρ=0.322, P=0.020).

Conclusions: Arterial hypertension stimulates the immune response regardless of blood pressure regulation status. Prolonged hypertension influences peripheral monocyte TLR4 expression and IL-17A serum levels. Anti-hypertensive drugs have different immunomodulatory effects: diuretics are associated with higher IL-17A concentration and beta-blockers with lower TLR4 expression.

Keywords: Adaptive immunity; Arterial hypertension; Innate immunity; Interleukin 17A; Toll-like receptor 4.

Publication types

  • Observational Study

MeSH terms

  • Adrenergic beta-Antagonists / pharmacology
  • Adrenergic beta-Antagonists / therapeutic use
  • Antihypertensive Agents / pharmacology
  • Antihypertensive Agents / therapeutic use
  • Arteries / physiopathology*
  • Case-Control Studies
  • Cross-Sectional Studies
  • Diuretics / pharmacology
  • Diuretics / therapeutic use
  • Humans
  • Hypertension / blood*
  • Hypertension / immunology
  • Immunity
  • Interleukin-17 / blood*
  • Monocytes / chemistry
  • Toll-Like Receptor 4 / analysis*

Substances

  • Adrenergic beta-Antagonists
  • Antihypertensive Agents
  • Diuretics
  • IL17A protein, human
  • Interleukin-17
  • TLR4 protein, human
  • Toll-Like Receptor 4