Chainy: an universal tool for standardized relative quantification in real-time PCR

Izaskun Mallona; Anna Díez-Villanueva; Berta Martín; Miguel A Peinado

doi:10.1093/bioinformatics/btw839

Chainy: an universal tool for standardized relative quantification in real-time PCR

Bioinformatics. 2017 May 1;33(9):1411-1413. doi: 10.1093/bioinformatics/btw839.

Authors

Izaskun Mallona¹, Anna Díez-Villanueva¹, Berta Martín¹, Miguel A Peinado¹

Affiliation

¹ Germans Trias i Pujol Health Science Research Institute (IGTP), Program for Predictive and Personalized Medicine of Cancer (IMPPC), Badalona 08916, Catalonia, Spain.

PMID: 28453678
DOI: 10.1093/bioinformatics/btw839

Abstract

Summary: Chainy is a cross-platform web tool providing systematic pipelines and steady criteria to process real-time PCR data, including the calculation of efficiencies from raw data by kinetic methods, evaluation of the suitability of multiple references, standardized normalization using one or more references, and group-wise relative quantification statistical testing. We illustrate the utility of Chainy for differential expression and chromatin immunoprecipitation enrichment (ChIP-QPCR) analysis.

Availability and implementation: Chainy is open source and freely available at http://maplab.cat/chainy.

Contact: imallona@igtp.cat.

Supplementary information: Supplementary data are available at Bioinformatics online.

MeSH terms

Chromatin Immunoprecipitation / methods*
Real-Time Polymerase Chain Reaction / methods
Real-Time Polymerase Chain Reaction / standards*
Reference Standards
Software*