Rapid and specific detection of Asian- and African-lineage Zika viruses

Nunya Chotiwan; Connie D Brewster; Tereza Magalhaes; James Weger-Lucarelli; Nisha K Duggal; Claudia Rückert; Chilinh Nguyen; Selene M Garcia Luna; Joseph R Fauver; Barb Andre; Meg Gray; William C Black 4th; Rebekah C Kading; Gregory D Ebel; Guillermina Kuan; Angel Balmaseda; Thomas Jaenisch; Ernesto T A Marques; Aaron C Brault; Eva Harris; Brian D Foy; Sandra L Quackenbush; Rushika Perera; Joel Rovnak

doi:10.1126/scitranslmed.aag0538

Rapid and specific detection of Asian- and African-lineage Zika viruses

Sci Transl Med. 2017 May 3;9(388):eaag0538. doi: 10.1126/scitranslmed.aag0538.

Authors

Nunya Chotiwan^{1

2}, Connie D Brewster¹, Tereza Magalhaes^{2

3}, James Weger-Lucarelli^{1

2}, Nisha K Duggal⁴, Claudia Rückert^{1

2}, Chilinh Nguyen^{1

2}, Selene M Garcia Luna^{1

2}, Joseph R Fauver^{1

2}, Barb Andre¹, Meg Gray^{1

2}, William C Black 4th^{1

2}, Rebekah C Kading^{1

2}, Gregory D Ebel^{1

2}, Guillermina Kuan⁵, Angel Balmaseda⁶, Thomas Jaenisch^{7

8}, Ernesto T A Marques^{3

9}, Aaron C Brault⁴, Eva Harris¹⁰, Brian D Foy^{1

2}, Sandra L Quackenbush¹, Rushika Perera^{1

2}, Joel Rovnak¹¹

Affiliations

¹ Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
² Arthropod-borne Infectious Disease Laboratories, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
³ Laboratory of Virology and Experimental Therapeutics, Centro de Pesquisas Aggeu Magalhaes, Fundacao Oswaldo Cruz, Recife-PE, Brazil.
⁴ Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA.
⁵ Centro de Salud Sócrates Flores Vivas, Ministry of Health, Managua, Nicaragua.
⁶ Laboratorio Nacional de Virología, Centro Nacional de Diagnóstico y Referencia, Ministry of Health, Managua, Nicaragua.
⁷ Section Clinical Tropical Medicine, Department for Infectious Diseases, Heidelberg University Hospital, Heidelberg, Germany.
⁸ German Centre for Infection Research (DZIF), partner site Heidelberg, Heidelberg, Germany.
⁹ Center for Vaccine Research, School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, USA.
¹⁰ Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, CA 94720-7360, USA.
¹¹ Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. joel.rovnak@colostate.edu.

Abstract

Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Culicidae
Humans
Nucleic Acid Amplification Techniques / methods*
RNA, Viral
Zika Virus

Substances

RNA, Viral

Abstract

Publication types

MeSH terms

Substances

Grants and funding