Abstract
Enzymatically prepared alginate oligomer (AO) promoted the growth of Chlamydomonas reinhardtii in a concentration-dependent manner. AO at 2.5 mg/mL induced increase in expression levels of cyclin A, cyclin B, and cyclin D in C. reinhardtii. CuSO4 at 100 μM suppressed the growth of C. reinhardtiin, and AO at 2.5 mg/mL significantly alleviated the toxicity of CuSO4. Increased intracellular reactive oxygen species level in C. reinhardtii induced by CuSO4 was reduced by AO. After cultivation with CuSO4 at 100 μM, expression levels of ascorbate peroxidase and superoxide dismutase in C. reinhardtii were increased, and AO reduced the increased levels of these enzymes. These results suggest that AO exhibits beneficial effects on C. reinhardtii through influencing the expression of various genes not only at normal growth condition but also under CuSO4 stress.
Keywords:
Chlamydomonas reinhardtii; CuSO4; alginate; alginate oligomer; gene expression.
MeSH terms
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Algal Proteins / genetics*
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Algal Proteins / metabolism
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Alginates / pharmacology*
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Antioxidants / pharmacology*
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Ascorbate Peroxidases / genetics
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Ascorbate Peroxidases / metabolism
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Cell Cycle / drug effects*
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Cell Cycle / genetics
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Chlamydomonas reinhardtii / drug effects*
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Chlamydomonas reinhardtii / genetics
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Chlamydomonas reinhardtii / growth & development
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Chlamydomonas reinhardtii / metabolism
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Copper Sulfate / antagonists & inhibitors
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Copper Sulfate / toxicity
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Cyclin A / genetics
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Cyclin A / metabolism
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Cyclin B / genetics
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Cyclin B / metabolism
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Cyclin D / genetics
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Cyclin D / metabolism
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Cytotoxins / antagonists & inhibitors
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Cytotoxins / toxicity
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Gene Expression Regulation / drug effects*
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Glucuronic Acid / pharmacology
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Hexuronic Acids / pharmacology
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Polymerization
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Reactive Oxygen Species / antagonists & inhibitors
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Reactive Oxygen Species / metabolism
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Superoxide Dismutase / genetics
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Superoxide Dismutase / metabolism
Substances
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Algal Proteins
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Alginates
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Antioxidants
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Cyclin A
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Cyclin B
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Cyclin D
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Cytotoxins
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Hexuronic Acids
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Reactive Oxygen Species
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Glucuronic Acid
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Ascorbate Peroxidases
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Superoxide Dismutase
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Copper Sulfate