Time-resolved cryo-electron microscopy: Recent progress

Joachim Frank

doi:10.1016/j.jsb.2017.06.005

Time-resolved cryo-electron microscopy: Recent progress

J Struct Biol. 2017 Dec;200(3):303-306. doi: 10.1016/j.jsb.2017.06.005. Epub 2017 Jun 16.

Author

Joachim Frank¹

Affiliation

¹ Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, 650 W. 168th Street, New York, NY 10032, United States; Department of Biological Sciences, Columbia University, United States. Electronic address: jf2192@columbia.edu.

Abstract

Time-resolved cryo-electron microscopy (cryo-EM) combines the known advantages of single-particle cryo-EM in visualizing molecular structure with the ability to dissect the time progress of a reaction between molecules in vitro. Here some of the recent progress of this methodology and its first biological applications are outlined.

Keywords: Cryo-EM; Microfluidics; RRF; Ribosome recycling; Short-lived states.

Publication types

Review
Research Support, N.I.H., Extramural

MeSH terms

Cryoelectron Microscopy / instrumentation*
Cryoelectron Microscopy / methods*
Equipment Design
Models, Molecular
Ribosomes / chemistry
Ribosomes / metabolism

Abstract

Publication types

MeSH terms

Grants and funding