[Effect of PDK1 on Neutrophil Differentiation]

Wei-Li Wang; Le Wang; Tian-Yuan Hu; Cong Li; Wei-Ping Yuan

doi:10.7534/j.issn.1009-2137.2017.03.053

[Effect of PDK1 on Neutrophil Differentiation]

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017 Jun;25(3):932-935. doi: 10.7534/j.issn.1009-2137.2017.03.053.

[Article in Chinese]

Authors

Wei-Li Wang¹, Le Wang¹, Tian-Yuan Hu¹, Cong Li¹, Wei-Ping Yuan²

Affiliations

¹ State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
² State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China. E-mail:wpyuan@ihcams.ac.cn.

PMID: 28641662
DOI: 10.7534/j.issn.1009-2137.2017.03.053

Abstract

Objective: To knock out PDK1 by using Vav-Cre and observe the effects of PDK1 knock out on the ratio, number and differentiation of neutrophil.

Methods: The PDK1 expression level of Vav-Cre;PDK1^lox/lox mouse in the bone marrow cells was analyzed by RT-PCR. The effect of PDK1 on hematopoietic progenitor was observed by CFU-C assay, and the effect of PDK1 on the ratio and number of neutrophil was detected by flow cytometric analysis.

Results: The RNA expression level of bone marrow cells of Vav-Cre;PDK1^lox/lox mouse was dramatically lower than that of the control mouse. The number of functional GMP was lower in Vav-Cre;PDK1^lox/lox mouse in contrast to controls. The percentage and number of neutrophil were lower, but the percentage of premyelocyte/myelocytes was more than 2 times in Vav-Cre;PDK1^lox/lox group compared with control groups.

Conclusion: PDK1 affects the process of the differention of hematopoietic stem cells to GMP, the neutrophil differention and maturation.

MeSH terms

Animals
Bone Marrow Cells
Cell Differentiation*
Gene Knockout Techniques
Hematopoietic Stem Cells
Mice
Neutrophils*
Protein Serine-Threonine Kinases / genetics*
Pyruvate Dehydrogenase Acetyl-Transferring Kinase

Substances

Pdk1 protein, mouse
Pyruvate Dehydrogenase Acetyl-Transferring Kinase
Protein Serine-Threonine Kinases