In Vivo Tracking of Edema Development and Microvascular Pathology in a Model of Experimental Cerebral Malaria Using Magnetic Resonance Imaging

J Vis Exp. 2017 Jun 8:(124):55334. doi: 10.3791/55334.

Abstract

Cerebral malaria is a sign of severe malarial disease and is often a harbinger of death. While aggressive management can be life-saving, the detection of cerebral malaria can be difficult. We present an experimental mouse model of cerebral malaria that shares multiple features of the human disease, including edema and microvascular pathology. Using magnetic resonance imaging (MRI), we can detect and track the blood-brain barrier disruption, edema development, and subsequent brain swelling. We describe multiple MRI techniques that can visualize these pertinent pathological changes. Thus, we show that MRI represents a valuable tool to visualize and track pathological changes, such as edema, brain swelling, and microvascular pathology, in vivo.

Publication types

  • Video-Audio Media

MeSH terms

  • Animals
  • Brain / blood supply
  • Disease Models, Animal
  • Edema / diagnostic imaging*
  • Edema / pathology
  • Humans
  • Magnetic Resonance Imaging / methods*
  • Malaria, Cerebral / diagnostic imaging*
  • Malaria, Cerebral / pathology
  • Mice