Production of a bisdemethylated microcystin variant by Planktothrix rubescens

Kristin Zoschke; Marta Schübel; Hilmar Börnick; Eckhard Worch

doi:10.1016/j.toxicon.2017.06.015

Production of a bisdemethylated microcystin variant by Planktothrix rubescens

Toxicon. 2017 Oct:137:95-98. doi: 10.1016/j.toxicon.2017.06.015. Epub 2017 Jun 29.

Authors

Kristin Zoschke¹, Marta Schübel², Hilmar Börnick², Eckhard Worch²

Affiliations

¹ Technische Universität Dresden, Institute of Water Chemistry, D-01062, Dresden, Germany. Electronic address: kristin.zoschke@tu-dresden.de.
² Technische Universität Dresden, Institute of Water Chemistry, D-01062, Dresden, Germany.

PMID: 28668563
DOI: 10.1016/j.toxicon.2017.06.015

Abstract

Reservoir samples from a bloom of Planktothrix rubescens were analyzed by ELISA and LC-MS/MS. The comparison of the results of both methods points to the presence of a microcystin variant not yet available as analytical standard and therefore, not detectable by LC-MS/MS analysis. It is proposed that the unknown cyanotoxin variant is a bisdemethylated microcystin variant, presumably [Asp³,Dha⁷]-microcystin-RR. [Asp³,Dha⁷]-MC-RR has not been described for a bloom of P. rubescens before.

Keywords: Cyanobacteria; Cyanotoxins; ELISA; LC-MS/MS; [Asp(3),Dha(7)]-microcystin-RR.

MeSH terms

Bacterial Toxins / analysis
Bacterial Toxins / chemistry*
Chromatography, Liquid / methods
Cyanobacteria / chemistry*
Fresh Water / chemistry*
Germany
Lakes
Marine Toxins
Microcystins / analysis
Microcystins / chemistry*
Tandem Mass Spectrometry / methods
Water Microbiology

Substances

Bacterial Toxins
Marine Toxins
Microcystins
microcystin RR