ROS mediated selection for increased NADPH availability in Escherichia coli

Biotechnol Bioeng. 2017 Nov;114(11):2685-2689. doi: 10.1002/bit.26385. Epub 2017 Aug 17.

Abstract

The economical production of chemicals and fuels by microbial processes remains an intense area of interest in biotechnology. A key limitation in such efforts concerns the availability of key co-factors, in this case NADPH, required for target pathways. Many of the strategies pursued for increasing NADPH availability in Escherichia coli involve manipulations to the central metabolism, which can create redox imbalances and overall growth defects. In this study we used a reactive oxygen species based selection to search for novel methods of increasing NADPH availability. We report a loss of function mutation in the gene hdfR appears to increase NADPH availability in E. coli. Additionally, we show this excess NADPH can be used to improve the production of 3HP in E. coli.

Keywords: 3HP production; E. coli; NAPDH; redox balance.

MeSH terms

  • Biological Availability
  • Citric Acid Cycle / physiology
  • Escherichia coli / physiology*
  • Gene Expression Regulation, Bacterial / physiology
  • Gene Expression Regulation, Enzymologic / physiology
  • Genetic Enhancement / methods*
  • Lactic Acid / analogs & derivatives*
  • Lactic Acid / isolation & purification
  • Lactic Acid / metabolism
  • Metabolic Engineering / methods*
  • NADP / biosynthesis*
  • Pentose Phosphate Pathway / physiology
  • Reactive Oxygen Species / metabolism*

Substances

  • Reactive Oxygen Species
  • Lactic Acid
  • NADP
  • hydracrylic acid