Hall effect biosensors with ultraclean graphene film for improved sensitivity of label-free DNA detection

Phan Thi Kim Loan; Dongqin Wu; Chen Ye; Xiaoqing Li; Vu Thanh Tra; Qiuping Wei; Li Fu; Aimin Yu; Lain-Jong Li; Cheng-Te Lin

doi:10.1016/j.bios.2017.07.045

Hall effect biosensors with ultraclean graphene film for improved sensitivity of label-free DNA detection

Biosens Bioelectron. 2018 Jan 15:99:85-91. doi: 10.1016/j.bios.2017.07.045. Epub 2017 Jul 19.

Authors

Phan Thi Kim Loan¹, Dongqin Wu², Chen Ye³, Xiaoqing Li³, Vu Thanh Tra⁴, Qiuping Wei⁵, Li Fu⁶, Aimin Yu⁷, Lain-Jong Li⁸, Cheng-Te Lin⁹

Affiliations

¹ Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo 315201, PR China; Physics Department, School of Education, Can Tho University, Can Tho City, Vietnam.
² Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo 315201, PR China.
³ Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo 315201, PR China; University of Chinese Academy of Sciences, 19A Yuquan Rd., Shijingshan District, Beijing 100049, PR China.
⁴ Physics Department, School of Education, Can Tho University, Can Tho City, Vietnam.
⁵ School of Materials Science and Engineering, Central South University, Changsha 410083, PR China.
⁶ College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, PR China.
⁷ Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Melbourne 3122, Australia.
⁸ Physical Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
⁹ Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo 315201, PR China; University of Chinese Academy of Sciences, 19A Yuquan Rd., Shijingshan District, Beijing 100049, PR China. Electronic address: linzhengde@nimte.ac.cn.

PMID: 28743083
DOI: 10.1016/j.bios.2017.07.045

Abstract

The quality of graphene strongly affects the performance of graphene-based biosensors which are highly demanded for the sensitive and selective detection of biomolecules, such as DNA. This work reported a novel transfer process for preparing a residue-free graphene film using a thin gold supporting layer. A Hall effect device made of this gold-transferred graphene was demonstrated to significantly enhance the sensitivity (≈ 5 times) for hybridization detection, with a linear detection range of 1pM to 100nM for DNA target. Our findings provide an efficient method to boost the sensitivity of graphene-based biosensors for DNA recognition.

Keywords: DNA hybridization; Gold-transfer method; Graphene biosensors; Hall effect measurement; Ultraclean surface.

MeSH terms

Biosensing Techniques*
DNA / chemistry
DNA / isolation & purification*
Gold / chemistry
Graphite / chemistry*
Limit of Detection
Nucleic Acid Hybridization
Surface Plasmon Resonance

Substances

Gold
Graphite
DNA