Advances in the analysis of "less-conventional" human body fluids: An overview of the CE- and HPLC-MS applications in the years 2015-2017

Electrophoresis. 2018 Jan;39(1):160-178. doi: 10.1002/elps.201700276. Epub 2017 Sep 1.

Abstract

Aim of this article is to focus the attention of the reader on the application of CE/MS and LC/MS to the analysis of human body fluids not currently used for the diagnosis of disorders and, for this reason, catalogued as "less/nonconventional" fluids, that is, tears, nasal secretions, cerumen, bronchoalveolar lavage fluid, sputum, exhaled breath condensate, nipple aspirate, breast milk, amniotic fluid, bile, seminal plasma, liposuction aspirate fluid, and synovial fluid. The pool of articles presented in this report demonstrates that, rather than being neglected, these fluids are an important resource for the evaluation of possible pathologic conditions. Thus, being a sort of mirror that reflects the normal internal characteristics and disease state of an individual, they benefit of an increasing appreciation. This review follows a previous report of this series and covers the latest developments in this field that have been published in specialist journals in the years 2015-2017.

Keywords: CE-MS; Human body fluids; LC-MS; Metabolomics; Proteomics.

Publication types

  • Review

MeSH terms

  • Animals
  • Body Fluids / chemistry*
  • Chemical Fractionation / methods
  • Chromatography, High Pressure Liquid / methods
  • Clinical Chemistry Tests / methods
  • Electrophoresis, Capillary / methods
  • High-Throughput Screening Assays / methods
  • Human Body
  • Humans
  • Mass Spectrometry / methods
  • Proteomics
  • Sensitivity and Specificity