H2-Driven biocatalytic hydrogenation in continuous flow using enzyme-modified carbon nanotube columns

Ceren Zor; Holly A Reeve; Jonathan Quinson; Lisa A Thompson; Thomas H Lonsdale; Frank Dillon; Nicole Grobert; Kylie A Vincent

doi:10.1039/c7cc04465h

H₂-Driven biocatalytic hydrogenation in continuous flow using enzyme-modified carbon nanotube columns

Chem Commun (Camb). 2017 Aug 31;53(71):9839-9841. doi: 10.1039/c7cc04465h.

Authors

Ceren Zor¹, Holly A Reeve², Jonathan Quinson¹, Lisa A Thompson², Thomas H Lonsdale², Frank Dillon³, Nicole Grobert³, Kylie A Vincent²

Affiliations

¹ Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Road, Oxford, OX1 3QR, UK. kylie.vincent@chem.ox.ac.uk and Department of Materials, University of Oxford, Parks Road, Oxford, OX1 3PH, UK.
² Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Road, Oxford, OX1 3QR, UK. kylie.vincent@chem.ox.ac.uk.
³ Department of Materials, University of Oxford, Parks Road, Oxford, OX1 3PH, UK.

PMID: 28795176
DOI: 10.1039/c7cc04465h

Abstract

We describe the implementation of a system of immobilised enzymes for H₂-driven NADH recycling coupled to a selective biotransformation to enable H₂-driven biocatalysis in flow. This approach represents a platform that can be optimised for a wide range of hydrogenation steps and is shown here for enantioselective ketone reduction and reductive amination.

MeSH terms

Amination
Biocatalysis*
Enzymes, Immobilized / metabolism*
Hydrogen / chemistry
Hydrogen / metabolism*
Hydrogenase / metabolism*
Hydrogenation
Ketones / chemistry
Ketones / metabolism
Nanotubes, Carbon / chemistry*
Oxidation-Reduction
Oxidoreductases / metabolism*

Substances

Enzymes, Immobilized
Ketones
Nanotubes, Carbon
Hydrogen
Oxidoreductases
Hydrogenase