Characterization of Macrophage Polarization States Using Combined Measurement of 2-Deoxyglucose and Glutamine Accumulation: Implications for Imaging of Atherosclerosis

Abstract

Objective: Despite the early promising results of ¹⁸F-fluorodeoxyglucose positron emission tomography for assessment of vessel wall inflammation, its accuracy in prospective identification of vulnerable plaques has remained limited. Additionally, previous studies have indicated that ¹⁸F-fluorodeoxyglucose uptake alone may not allow for accurate identification of specific macrophage activation states. We aimed to determine whether combined measurement of glucose and glutamine accumulation-the 2 most important bioenergetic substrates for macrophages-improves the distinction of macrophage inflammatory states and can be utilized to image atherosclerosis.

Approach and results: Murine peritoneal macrophages (MΦ) were activated ex vivo into proinflammatory states with either lipopolysaccharide (MΦ_LPS) or interferon-γ+tumor necrosis factor-α (MΦ_{IFN-γ+TNF-α}). An alternative polarization phenotype was induced with interleukin-4 (MΦ_IL-4). The pronounced increase in 2-deoxyglucose uptake distinguishes MΦ_LPS from MΦ_{IFN-γ+TNF-α}, MΦ_IL-4, and unstimulated macrophages (MΦ₀). Despite having comparable levels of 2-deoxyglucose accumulation, MΦ_IL-4 can be distinguished from both MΦ_{IFN-γ+TNF-α} and MΦ₀ based on the enhanced glutamine accumulation, which was associated with increased expression of a glutamine transporter, Slc1a5. Ex vivo autoradiography experiments demonstrated distinct and heterogenous patterns of ¹⁸F-fluorodeoxyglucose and ¹⁴C-glutamine accumulation in atherosclerotic lesions of low-density lipoprotein receptor-null mice fed a high-fat diet.

Conclusions: Combined assessment of glutamine and 2-deoxyglucose accumulation improves the ex vivo identification of macrophage activation states. Combined ex vivo metabolic imaging demonstrates heterogenous and distinct patterns of substrate accumulation in atherosclerotic lesions. Further studies are required to define the in vivo significance of glutamine uptake in atherosclerosis and its potential application in identification of vulnerable plaques.

Keywords: animals; energy metabolism; glucose; inflammation; mice.