Antioxidant protection by β-selenoamines against thioacetamide-induced oxidative stress and hepatotoxicity in mice

J Biochem Mol Toxicol. 2017 Dec;31(12). doi: 10.1002/jbt.21974. Epub 2017 Aug 11.

Abstract

Thioacetamide (TAA) is a hepatotoxin that rapidly triggers the necrotic process and oxidative stress in the liver. Nevertheless, organic selenium compounds, such as β-selenoamines, can be used as pharmacological agents to diminish the oxidative damage. Thus, the aim of this study was to investigate the protective effect of the antioxidant β-selenoamines on TAA-induced oxidative stress in mice. Here, we observed that a single intraperitoneal injection of TAA (200 mg/kg) dramatically elevated some parameters of oxidative stress, such as lipid peroxidation and reactive oxygen species (ROS) production, as well as depleted cellular antioxidant defenses. In addition, TAA-induced edema and morphological changes in the liver, which correlate with high serum aspartate and alanine aminotransferase enzyme activities, and a decrease in cell viability. Conversely, a significant reduction in liver lipid peroxidation, ROS production, and edema was observed in animals that received an intraperitoneal injection of β-selenoamines (15.6 mg/kg) 1 h after TAA administration.

Keywords: liver damage; organic selenium compounds; oxidative stress; selenol; thioacetamide.

MeSH terms

  • Amines / pharmacology
  • Animals
  • Antioxidants / pharmacology*
  • Chemical and Drug Induced Liver Injury / metabolism
  • Chemical and Drug Induced Liver Injury / prevention & control*
  • Drug Evaluation, Preclinical
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • Glutathione Transferase / metabolism
  • Lipid Peroxidation
  • Liver / drug effects*
  • Liver / enzymology
  • Liver / pathology
  • Male
  • Mice
  • Organoselenium Compounds / pharmacology*
  • Oxidative Stress / drug effects*
  • Reactive Oxygen Species / metabolism
  • Thioacetamide

Substances

  • Amines
  • Antioxidants
  • Organoselenium Compounds
  • Reactive Oxygen Species
  • Thioacetamide
  • Glutathione Peroxidase
  • Glutathione Transferase
  • Glutathione